In our study, we achieved sufficient statistical power to demonstrate a link between high levels of EBV DNA in blood and subsequent occurrence of systemic B lymphoma. However, the sensitivity and specificity yielded by the different levels of the EBV load cut-off were Compound Library screening not optimal; therefore, at this stage EBV load does not have major clinical relevance in this context. Even if we could demonstrate an association between EBV DNA load and progression to systemic B lymphoma, EBV DNA load values
overlapped between cases and controls and the best cut-off value (> 3.2 log10 copies/106 PBMCs) had a sensitivity and specificity of only 75 and 65%, respectively. Other innovative methods should be assessed for improved prediction of the risk of lymphoma, particularly among high-risk HIV-infected see more patients such as those with persistent HIV replication or decreasing CD4 cell counts. However, in this study, patients with undetectable EBV DNA in PBMCs did not develop NHL, while an increased EBV blood load was associated with systemic B lymphoma. Therefore, a high EBV DNA blood level in high-risk HIV-infected patients should alert clinicians to a greater possibility of developing NHL. This study was supported by the Agence Nationale de recherches sur le Sida et les
hépatites virales (ANRS). Author contributions: The contribution of all authors was essential. ML-V, JMS and PM coordinated the EBV PCR tests and were responsible for the quality results for these real-time PCR tests. ML-V, RS and LM were responsible for study design, data analysis, data interpretation and manuscript preparation.
FB, CG, CR, PM and JMS participated in data interpretation. RS and LM were responsible for statistical analysis. RS, FB, CD and LM were responsible for data collection. All authors have seen and approved the final version of the paper. Conflicts of interest: None of the authors has any financial or personal relationship with people or organizations that could CHIR99021 inappropriately influence this work, although most members of the group have received financial support from a variety of pharmaceutical companies for research, travel grants or speaking engagements. “
“The pharmacokinetics (PK) of antiretrovirals (ARVs) in older HIV-infected patients are poorly described. Here, the steady-state PK of two common ARV regimens [tenofovir (TFV)/emtricitabine (FTC)/efavirenz (EFV) and TFV/FTC/atazanavir (ATV)/ritonavir (RTV)] in older nonfrail HIV-infected patients are presented. HIV-infected subjects ≥55 years old not demonstrating the frailty phenotype were enrolled in an unblinded, intensive-sampling PK study. Blood plasma (for TFV, FTC, EFV, ATV and RTV concentrations) and peripheral blood mononuclear cells [PBMCs; for tenofovir diphosphate (TFV-DP) and emtricitabine triphosphate (FTC-TP) concentrations] were collected at 11 time-points over a 24-hour dosing interval.