\n\nPatients and methods: We tested the association PLX4032 in vitro between smoking status and inhibition of platelet aggregation (IPA) in response to a clopidogrel loading of 600-mg in 20 healthy

subjects. We then enrolled 138 consecutive STEMI patients treated with primary coronary stenting. On-clopidogrel platelet reactivity (PR) was assessed with conventional aggregometry and the VerifyNow P2Y(12) assay, according to smoking status.\n\nResults: After 6 hours post-loading in healthy subjects, CS patients on >= 10 cigarettes/day showed a significantly higher value of 5 mu mol/L ADP-stimulated IPA (P=0.006), and had a trend toward a greater value of 20 mu mol/L ADP-stimulated IPA (P=0.093) compared with non-smokers.

In STEMI patients, there was no difference in PR between non-smokers (n=66) and CS patients<10 cigarettes/day (n=16). CS patients on >= 10 cigarettes/day (n=56) demonstrated lower PR with 5 and 20 mu mol/L ADP (40.9 +/- 16.1% versus 46.6 +/- 11.7%, P=0.028, and 53.8 +/- 16.6% versus 59.2 +/- 12.2%, P = 0.040, respectively) and lower P2Y12 reaction units (204 +/- 85 versus 270 +/- 69, P<0.001) than non-smokers. On multivariate analyses, CS >= 10 cigarettes/day was the only predictor of low on-clopidogrel PR (<= 33%; the lowest quartile of 5 mu mol/L ADP-induced PR; odds ratio 4.651, 95% confidence interval 1.181-18.519, ERK inhibitor in vitro P=0.028).\n\nConclusion: CS seems to increase antiplatelet

response to clopidogrel in healthy volunteers and STEMI patients. Smoking 10 or more PXD101 cigarettes/day can significantly decrease on-clopidogrel platelet reactivity in these populations. (C) 2010 Elsevier Ltd. All rights reserved.”
“FT-IR and FT-Raman spectra of (E)-N-carbamimidoyl-4-((3,4-dimethoxybenzylidene) amino) benzenesulfonamide were recorded and analyzed. The vibrational wavenumbers were computed using HF/6-31G*, B3PW91/6-31G* and B3LYP/6-31G* basis. The data obtained from vibrational wavenumber calculations are used to assign vibrational bands obtained experimentally. The results indicate that the B3LYP method is able to provide satisfactory results for predicting vibrational frequencies and structural parameters. The calculated first hyperpolarizability is comparable with the reported values of similar derivatives and is an attractive object for future studies of non-linear optics. The geometrical parameters of the title compound are in agreement with that of similar derivatives. (C) 2011 Elsevier B.V. All rights reserved.”
“Progress in the quantum mechanics of biological molecules is being driven by computational advances. The notion of quantum kernels can be introduced to simplify the formalism of quantum mechanics, making it especially suitable for parallel computation of very large biological molecules.