, 1998). Normally distributed data are presented as mean ± SE. Estrogen antagonist Non-normally distributed data are presented as median and interquartile ranges (IQR). For analysis, non-normally distributed data were logarithmically transformed (Hussain et al., 2011). For threshold loading runs, physiologic data were analyzed at five points in time: start and end of loading, and three periods taken at equal time intervals between start and end of loading. Measurements were obtained from 5 to 10 consecutive

breaths at each point. Data at the five time periods were compared by one-way analysis of variance (ANOVA) with repeated measures. Adjustments for multiple comparisons were made with the Sidak method when appropriate. Pearson’s correlation coefficient (r) was used to detect correlation among variables. Statistical tests were 2-sided. p ≤ 0.05 was considered significant. The 17 subjects sustained loading for 7.8 ± 0.7 min. Fourteen stopped because of unbearable air hunger – either alone or in combination with unbearable breathing effort. Three stopped mainly because of unbearable Small molecule library breathing effort. PETCO2 increased in all subjects between the start and end of loading (p < 0.0005) ( Fig. 3). Likewise, global inspiratory effort – quantified as tidal change in airway pressure (ΔPaw) – increased in all subjects between the start

and end of loading (p < 0.0005) ( Fig. 3). Despite the increase in effort, tidal volume (VT) decreased (p < 0.003). Over the course of loading, both ΔPdi and ΔEAdi increased (p < 0.0005) ( Fig. 4). The relative increase in ΔPdi was greater than the relative increase in ΔEAdi. Accordingly, neuromechanical coupling (ΔPdi/ΔEAdi) increased over the course of loading (p ≤ 0.005) ( Fig. 4). At task failure, ΔEAdi was 74.9 ± 4.9%

of maximum. Neuromechanical coupling recorded while subjects sustained the small, threshold load (−20 cm H2O) just before the incremental loading Nintedanib (BIBF 1120) (Fig. 2) was 0.68 ± 0.07 cm H2O. Immediately after task failure, coupling increased to 0.80 ± 0.07 cm H2O (p < 0.004, ANOVA); 10 min and 30 min later, coupling had returned to baseline values: 0.66 ± 0.05 and 0.64 ± 0.07 cm H2O. Incremental threshold loading caused a progressive increase in IC, extradiaphragmatic muscle contribution to tidal breathing (ΔPga/ΔPes), expiratory muscle recruitment (expiratory rise in Pga), and rate of transdiaphragmatic pressure development (ΔPdi/TI) (p ≤ 0.007 all instances) ( Fig. 5). The progressive increase in IC – mirroring decrease in EELV – was related to improvement in diaphragmatic neuromechanical coupling (ΔPdi/ΔEAdi) (R2 = 0.88). Inspiratory loading triggered phasic electrical activity of the lower abdominal muscles during exhalation that increased as loading progressed (Fig. 6). This electrical activity continued at end-exhalation, and was followed by phasic electrical activity during neural inhalation (p ≤ 0.0008 in all instances).