1H NMR spectra were registered on a Bruker (Rheinstetten, Germany) DRX-500 instrument operating at 500.13 MHz for 1H observations using a Broadband Inverse (BBI) microprobe maintained at 298 K. Suppression of the H2O signal was obtained using pre-saturation experiment (pulse program zgcppr). In this case, 1H NMR spectra were

digitized into 16K data points over a spectral width of 20 ppm with an acquisition time of 1.8 s. An additional relaxation delay of 10 s was included, making a total recycling time of 11.8 s. A 90° pulse was used with 32 scans. Spectra were Fourier transformed applying a line broadening apodization function of 2.0 Hz. Double suppression of the DMSO and the residual H2O signals was obtained using pre-saturation experiment (pulse program Wetdc). JAK inhibitor In this

case, 1H NMR spectra were digitized into 32 K data points over a spectral width Bleomycin of 15 ppm with an acquisition time of 1.1 s. An additional relaxation delay of 5 s was included, making a total recycling time of 6.1 s. A 90° pulse was used with 8 scans. Spectra were Fourier transformed applying a line broadening apodization function of 1.0 Hz. All NMR spectra were processed in Bruker TopSpin 1.3. Chemical shifts are referenced to the internal standard TSP at 0.0 ppm present in each sample at the concentration of 0.58 mM. All spectra were manually phased and baseline corrected. Normalized dose–response curves of single chemicals and binary mixtures were fitted to sigmoidal shape curves with values between 0 and 1 (0–100%) by using five different theoretical models. Subsequently the two 4-Aminobutyrate aminotransferase classical approaches to mixtures

study, CA and IA, have been applied to each of the used theoretical models to compare calculated and experimental results from binary mixtures dose–response curves. Several models have been proposed in literature (Backhaus et al., 2004), of which we applied: – Weibull (W): equation(1) f(x)=exp[−exp(θ1+θ2log10 x)]f(x)=exp[−exp(θ1+θ2log10 x)]- Box–Cox transformed Weibull (BCW): equation(2) f(x)=exp−expθ1+θ2xθ3−1θ3- logit (L): equation(3) f(x)=1−11+exp(−θ1−θ2log10x)- Generalized logit (GL): equation(4) f(x)=1−1[1+exp(−θ1−θ2log10x)]θ3- Morgan-Mercier Flodin (MMF): equation(5) f(x)=11+θ1 xθ3where θ1, θ2,and θ3 are parameters of the equations. Eqs. (1), (2), (3), (4) and (5) only consider one type of effect, i.e. the response (the mean firing rate) decreases as the dose increases. However, in some cases, we could observe a bi-phasic behavior: an excitatory effect at low concentrations followed by an inhibitory effect at higher concentrations. In this case, it is possible to use a function developed by Beckon et al. (2008), which has the following form: equation(6) f(x)=11+(εup/x)βup11+(εdn/x)βdnwith βup > 0 and βdn < 0. Following Beckon et al. (2008) the β-values represent the steepness, whereas ɛ-values represent the dose at the mid-point of the rising and of the falling respectively.