, 2002, Clayton and Byrne, 1993 and Yao and Byrne, 1998). Because pH measurements based on sulfonephthalein indicators are highly reproducible and calibration-free, these methods obviate some of the disadvantages associated with the use of pH-sensitive glass electrodes (e.g., requirements for frequent calibration (Dickson, 1993)). Spectrophotometric measurements of seawater pH (Byrne and

Breland, 1989, Clayton and Thiazovivin Byrne, 1993 and Robert-Baldo et al., 1985) are based on observations of absorbance (A) contributions from distinctly colored acidic (HL−) and basic (L2 −) forms of pH-sensitive indicator dyes (generally diprotic sulfonephthalein indicators) that are dissolved in seawater at low concentrations: equation(1)

HL−⇔H++L2−.HL−⇔H++L2−. Seawater pH on the total hydrogen ion concentration scale (Byrne and Breland, 1989, Byrne et al., 1988, Clayton and Byrne, 1993 and Robert-Baldo et al., 1985) is obtained using Eq. (2): equation(2) pHT=−logK2Te2+log[R−e1/1−R·e3/e2]where K2T is the equilibrium constant for Eq.  (1); R is the ratio of sulfonephthalein absorbances at λ1 and λ2, the wavelengths of maximum absorbance of the L2 − and HL− forms of the indicator (i.e., R = λ2AL2 − / λ1AHL−); and the ei coefficients are ratios of indicator molar absorptivities (ε) at wavelengths λ1 and λ2: equation(3) e1=λ2εHL–λ1εHL–;e2=λ2εL2–λ1εHL–;e3=λ1εL2–λ1εHL–. Sulfonephthalein indicators (e.g., cresol red, bromocresol purple, thymol blue) have been used for high-precision measurements of pH, total alkalinity, and total dissolved inorganic carbon in seawater and Venetoclax cost freshwater (Byrne et al., 2002, Byrne and Breland, 1989, Clayton and Byrne, 1993, Hopkins et al., 2000, Robert-Baldo et al., 1985, Yao and Byrne, 1998, Yao and Byrne, 2001 and Zhang and Byrne, 1996). The indicator meta-cresol purple (mCP) is often used because its ideal indicating range (7.2 ≤ pHT ≤ 8.1)

Reverse transcriptase provides good coverage of the pH ranges typically encountered in a variety of saltwater environments (Byrne et al., 1988 and Clayton and Byrne, 1993). Since indicator impurities can contribute to pH offsets as large as 0.018 pH units (Liu et al., 2011, Patsavas et al., 2013 and Yao et al., 2007), procedures to purify commercially available indicator powders have been developed and the properties of some purified indicators, including mCP, have been reported (Liu et al., 2011 and Patsavas et al., 2013). The precision of spectrophotometric pH measurements is on the order of ± 0.0004 (Byrne et al., 1999, Clayton and Byrne, 1993 and Liu et al., 2011), in accord with the requirements of open-ocean and laboratory studies of ocean acidification. Solution pH also serves as an important water quality parameter in monitoring programs associated with coastal zone, aquaculture, and aquarium management.