3.1. Ten Repetitions Maximum Testing (10RM)A light warmup with ten submaximal repetitions was instructed with a two-minute rest before the initiation of the tests. during To obtain a reliable 10RM load, data was assessed during two nonconsecutive days in the 45�� leg press (LP) (Cybex International, Medway, MA). The 10RM load was determined by using the maximum weight that could be lifted for ten consecutive repetitions at a constant velocity of 3 seconds per repetition (1.5s in concentric and 1.5s in the eccentric phase). If the subject could not accomplish the 10RM in the first attempt, the weight was adjusted by 4�C10kg. Each subject performed a maximum of five 10RM attempts with 5-minute rest intervals between attempts. To minimize the error during tests, the following strategies were adopted according to Sim?o et al.
[13]: (a) standardized instructions concerning the testing procedure were given to the participants before the test; (b) participants received standardized instructions on exercise technique; (c) verbal encouragement was provided during the testing procedure; (d) the mass of the leg press sled was considered. A paired student t-test did not show significant differences between the 10RM tests (intraclass correlation coefficient r = 0.93, P �� 0.05). The heaviest load achieved was considered the 10RM.3.2. Resistance Training SessionPrior to the RT session, volunteers rested quietly in a seated position for 20 minutes to facilitate baseline measurements of systolic blood pressure (SBP), diastolic blood pressure (DBP), and heart rate (HR).
Before the session, a specific warmup with 1 set of 10 repetitions at 50% of 10RM was performed. Subsequently, the RT was initiated, and volunteers performed three sets, of 10 repetitions GSK-3 at 80% of 10RM in the 45�� leg press, with 30s rest interval between sets. No pauses between concentric and eccentric actions were allowed, and investigators were present to assure that standardized exercise technique was maintained. Volunteers were instructed to avoid the Valsalva maneuver during the RT session [1] in order to minimize potentially abrupt modifications in the cardiovascular response with exercise. A preliminary orientation to establish appropriate weight loads and instruct the participant on proper lifting techniques, range of motion of the leg press exercise, and correct breathing patterns were taken to avoid Valsalva maneuver. During sets BP and HR were measured between the last two sets by the end of the concentric phase of the contractions and in the postexercise period (5 and 30 minutes after). All BP measurements (pre- and postsession) were obtained using the auscultatory method with appropriate cuffs fitted according to the size of the upper arm of each participant.