96 degrees, with no significant differences between slitlamp and digital photograph measurements. There was a trend toward better mesopic contrast sensitivity with glare in the toric IOL group. There were no differences in VF-14 or patient satisfaction results; 15% of patients in the toric IOL group and 45% in the relaxing-incision group required distance spectacles postoperatively.
CONCLUSION: Although refractive astigmatism decreased in both groups, toric IOL implantation was more effective and predictable, resulting in greater spectacle independence.”
“Background: Multiplex cytokine profiling systems are useful tools for investigating correlates of protective immunity. Several Luminex
and flow cytometry methods 4SC-202 nmr are commercially available but there is limited information on the relative
performance of different kits. A series of comparison experiments were carried out to determine the most appropriate method for our subsequent studies.
Methods: Two Luminex methods were compared, the Bio-Rad human 17-plex panel and the Invitrogen (formerly BioSource) human cytokine 10-plex kit, and two flow cytometry methods, the Becton Dickinson Human Th1/Th2 Cytokine Kit (CBA) and the Bender MedSystems Human Th1/Th2 11plex FlowCytomix Multiplex Kit. All kits were tested for the measurement of cytokines in supernatants collected from human leukocytes stimulated with viable Plasmodium falciparum infected red blood cells (iRBC) or P. falciparum schizont lysates.
Results: Data indicated that the kits differed in sensitivity and reproducibility depending on the cytokine, and detected different quantities 3-MA price Screening Library price of some cytokines. The Bio-Rad 17-plex kit was able to detect more positive responses than the Invitrogen 10-plex kit. However, only when detecting IL-1, IL-6 or TNF did the two Luminex based methods correlate with one another. In this study, the flow cytometry based techniques were less variable and correlated better with one another. The two flow cytometry based kits showed significant correlation when detecting IFN-gamma, IL-2, TNF,
IL-10 and IL-6, but overall the BD kit detected more positive responses than the Bender MedSystems kit.
Conclusions: The microsphere suspension array technologies tested differed in reproducibility and the absolute quantity of cytokine detected. Sample volume, the number of cytokines measured, and the time and cost of the assays also differed. These data provide an accurate assessment of the four techniques, which will allow individual researchers to select the tool most suited for their study population.”
“We propose here, a comprehensive model for the solid-state polymerization (SSP) of a low to moderate molecular weight (MW) prepolymer of lactic acid, to produce high MW poly(L-lactic acid) (PLLA). The reactions are rationally assumed to occur only in the amorphous region, and effective concentrations of end groups, vary with crystalinity, X-c, during SSP.