All the facial lacerations underwent thorough debridement as below. Cleaning and disinfection In order to release the pain of patients, local anesthetic was administrated before wounds cleaning.

After covering with sterilized dressing to the wounds, aseptic carbasus was used to scrub the area around the wounds 2-3 times with 20% liquid soap and water. Subsequently, the wounds were alternating douched with 20% liquid soap and physiological saline, and then 3% hydrogen peroxide and physiological saline. The total cleaning time was not less than 15 minutes each wound. A great quantity of 0.05% iso-osmia Inhibitors,research,lifescience,medical iodophors (1 portion 0.5% iodophors stock solution + 9 portion physiological saline) was used to disinfect the wounds, not less than 5 minutes. Caution, during the whole cleaning and disinfection Inhibitors,research,lifescience,medical procedure, the interior part of the wounds was more important than the surface of the wounds. Debridement All the inactivated tissues, coagulated blood, foreign material and serious contaminated tissues were carefully removed to MLN0128 in vitro expose surrounding healthy tissue. It was essential to remain their integrity as far as possible, so as to be repaired afterwards. The last procedure of debridement was douched

the inside part of laceration with 0.05% iodophors again, the sterile gloves, aseptic covers and surgical instruments was Inhibitors,research,lifescience,medical prepared for tissue repair. At this time, passive immunity, if necessary, should be given (Rabies Immunoglobulin or Rabies Antiserm). Regarding the importance of impaired facial organ or tissue, it was essential to remain their integrity which could be repaired afterwards. Important tissue repair All the important impaired or missing facial Inhibitors,research,lifescience,medical organ or tissues Inhibitors,research,lifescience,medical (such as eyelid, eyeball, nasolacrimal canal, parotid, nose, ear etc) were repaired with a suitable operation after the lacerations reached clinical healing. Wound closure After

thorough cleaning and debridement, the laceration was left open in group A; while those in group B was closed immediately. The 5/0 or 6/0 stylolite was used. All the patients were administrated rabies prophylactic active Cell press immunity and/or passive immunity according to Rabies Exposure Prophylactic and Handle Working Standard (2009 edition). Tetanus antitoxin (TAT) was given, if necessary. Drainage was carried out as the actual condition of laceration. Drain was placed innermost of the wound and replaced or pulled out according to the drainage quantity, usually 24h-48h after operation. All the wounds were covered with sterilized dressing and changed dressings 24h-48h after operation. The stitches in group B was removed 5d-7d after operation according to the wound healing condition. Antibiotic was used only after the wounds infection taking place.