The tats Chliche molecular weight of a bis-indole alkaloids in combination with EI-MS ASA404 DMXAA com information about the structure of these complex molecules have been developed. This strategy of using chemical ionization with methane as the reagent gas and EI-MS of Ma Measures used in the characterization of the VLB, leurosine, vincovaline andepi vincovaline w During their enantioselective synthesis. Typically, CI, FD and FAB-ionization soft Ma took For MS molecular weight determination or for high res Send measurements and get the elemental composition of molecules, in certain cases Cases have been applied, the fragments were also cited, but without most of the time any explanation tion serves only to the NMR-based Strukturaufkl support tion.
In an informative study that these soft ionization techniques have k Can also show detailed structural information, several new metabolites of vinorelbine by the erg Nzenden direct use of COM-FAB-MS Dihydrofolate Reductase cancer and NMR measurements have been identified. One of the metabolites showed a peak of more than quasi-molecular ions ions relative to vinorelbine and provided realistic character of ion peaks mzand wasDa fragments. Vinorelbine as fragment ions mzandfor fractions velbanamine and vindoline, where, in each case, one can Close S that the section has been oxidized by vinorelbine velbanamine. NMR analysis best The preferential hydroxylation of the carbon atom to an aromatic carbon. On the other hydroxylated metabolites, a comparison of the spectrum with the C MSMS OH vinorel Bine and vinorelbine itself, the hydroxyl group at C-atoms of C, C and DC are best shown by NMR analysis Preferential dihydroxylation hydroxide C.
In this case, the application MSMS was ma decisive for the identification of metabolites that could not be obtained as a mixture. ESI-MS analysis Our literature search AZD1152-HQPA identified only one article in which the ESI-MS fragmentation mechanism of bis-indole alkaloids from that VLB and VCR, is discussed. ESI mass spectra of VLB and VCR were recorded on a mass spectrometer ion trap solution with low Aufl. Fig.shows structures putative fragments main features described by the authors. The mechanism of fragmentation and molecular structures of the fragments were analogous to the results of the governments of the high res Send EI-MS measurement w S were carried out during the proposed. K and O fragments are analogues of fragment B and J, respectively.
Fragment N is the analogue of G EI-MS fragment by rearranging familiar retro-Diels-Alder reaction is obtained. By implementing fragment The fragment above mechanism is exclusively Prevented primarily by the loss of a C CHCOOH produced situation in our own work to new VLB-and VCR-contamination, a deep were identified HighRes send Tandem MS analysis of VLB and VCR were able to demonstrate carried out and based on MSN experiments we find that the nominal allele CHCOOH loss, in a hnlichen report the loss of HCOOCH also of C occur This observation k nnte be used if we have to contamination by VLB LC MSMS line in which the structural changes involved are identified, the Group C COOMe have to. High precision in our study Send ESI MSMS we could rationalize the formation of several other fragments. Fig.shows the high res Send ESI-MS spectrum of the LTQ FT mass spectrometer on a VLB ultra recorded in our laboratory. Thanks to our s