Bacillus cereus group and Staphylococcus species may produce toxins and cause foodborne illness.
Significance and Impact of the Study:
The results of this study provide fundamental information that may be used to enhance the microbial quality and safety of kava beverages.”
“Aims:
To examine plant terpenoids as inducers of TCE (trichloroethylene) biotransformation by an indigenous this website microbial community originating from a plume of TCE-contaminated groundwater.
Methods and Results:
One-litre microcosms of groundwater were spiked with 100 mu mol 1-1 of TCE and amended weekly for 16 weeks with 20 mu l 1-1 of the following plant monoterpenes:
linalool, pulegone, R-(+) carvone, S-(-) carvone, farnesol, cumene. Yeast extract-amended and unamended control treatments were also prepared. The addition of R-carvone and S-carvone, linalool and cumene resulted in the biotransformation of upwards of 88% of the TCE, significantly more
than the unamendment control (61%). The aforementioned group www.selleckchem.com/products/gs-9973.html of terpenes also significantly (P < 0 center dot 05) allowed more TCE to be degraded than the remaining two terpenes (farnesol and pulegone), and the yeast extract treatment which biotransformed 74-75% of the TCE. The microbial community profile was monitored by denaturing gradient gel electrophoresis and demonstrated much greater similarities between the microbial communities in terpene-amended treatments than in the yeast extract or unamended controls.
Conclusions:
TCE biotransformation can be significantly enhanced through the addition of selected plant terpenoids.
Significance and Impact of the Study:
Plant terpenoid and nutrient supplementation to groundwater might provide an environmentally benign means of enhancing the rate of in situ TCE bioremediation.”
“Aims:
To compare responses of a soil bacterium to Cu and Cd.
Methods and Results:
In minimal
medium, Cd caused a dose-dependent growth stasis of logarithmic phase cells of Pseudomonas putida, strain KT2440, whereas Cu did not compromise growth up to 10 mg l-1. Proteomics showed changes in accumulation of both membrane and soluble proteins by 6 h of treatment; increased Krebs cycle enzymes were apparent. Transcript analysis showed Cd- and Cu-induced different (-)-p-Bromotetramisole Oxalate genes. Cd-induced genes encoding the transcriptional regulator CzrR2; an outer membrane protein associated with lipopolysaccharide stability, H1; two oxidative stress protective proteins and the P-type ATPase, CadA2, associated with Cd2+ efflux. The genes most responsive to Cu encoded the regulator CopR1 and the outer membrane resistance protein regulated by CopR1, CopB1; a putative porin, PorD and the Cu-binding protein, PacZ or CopZ, and CopA2.
Conclusions:
These findings support that a soil pseudomonad restricts internalization of the metals by using different sets of binding proteins and efflux pumps.