Cluster analysis was performed Evofosfamide supplier using UPGMA algorithm of the Bionumerics

v. 4.6 software, with a cutoff value set at 85%. Numbers of repeats are showed in each MLVA marker. The number -2.0 was assigned if no PCR product could be amplified. Hemolysis in agar plate containing 5% sheep blood. Phenotypic and genotypic characterization of antimicrobial susceptibility All isolates were susceptible to penicillin, ampicillin, cefepime, cefotaxime, chloramphenicol, levofloxacin and vancomycin. Resistance to erythromycin and clindamycin was detected in 16 (19.3%) and 11 (13.3%) isolates, respectively. All isolates resistant to clindamycin were also resistant to erythromycin, and among them only

one had a constitutive macrolide-lincosamide-streptogramin B (cMLSB) phenotype (minimal inhibitory concentration – MIC > 8.0 μg/mL for both antimicrobials) and harbored the ermB gene. Of the 10 isolates displaying the indutible MLSB (iMLSB) phenotype, seven carried the ermA gene, whereas one isolate carried the ermB gene and two both genes. All isolates (n = 5) resistant only to erythromycin showed phenotype M and carried the mefA/E gene. Resistance to both erythromycin and clindamycin was detected among isolates belonging to serotypes V (n = 7) and III (n = 4), which were grouped in MTs 1, 3, 4, 6 and 7. All isolates resistant only to erythromycin belonged to serotype Ia and MT8 (Table 1). Table 1 Macrolide/lincosamide resistant Streptococcus agalactiae : distribution of capsular type, MLVA genotypes and antimicrobials resistance features OSI-906 price Isolate Source MLVA Genotypesa Capsular typeb Erythromycin resistance phenotypec Erythromycin Ibrutinib price resistance genesd MIC (μg/mL)e           ermA ermB mefA/E DA E 15 Urine 8 Ia M – - + 0.06 4.0 22 Urine 8 Ia M – - + 0.06 4.0 46 Urine 8 Ia M – - + 0.06 4.0 120 Urine 8 Ia M – - + 0.06 4.0 121 Swab 8 Ia M – - + 0.03 2.0 66 Urine 1 III iMLSB – + – 0.06 2.0 109 Urine 1 III iMLSB + – - 0.03 2.0 113 Urine

1 III iMLSB + + – 0.03 2.0 114 Urine 1 III iMLSB + – - 0.06 > 8.0 65 Urine 4 V iMLSB + – - 0.06 4.0 105 Urine 3 V iMLSB + – - 0.06 8.0 108 Urine 6 V iMLSB + – - 0.06 8.0 112 Urine 6 V iMLSB + – - 0.06 4.0 115 Swab 7 V cMLSB – + – > 8.0 > 8.0 116 Swab 4 V iMLSB + + – 0.06 8.0 117 Urine 6 V iMLSB + – - 0.06 4.0 aThe genetic diversity was assessed by MLVA typing [32]. A cutoff value of 85% similarity was CH5183284 chemical structure applied to define MLVA types. bThe capsular type was identified by multiplex-PCR [43]. cErythromycin resistance phenotype was determined by the double-disk diffusion method [46]. dThe presence of specified gene was determined by PCR. (+) Presence; (-) Absence. eThe minimum inhibitory concentrations (MIC) were determined by the agar-dilution method. Clindamycin (DA); Erythromycin (E).