Finally, cells from the non tumorigenic cell line HaCaT were less sensitive with the different treatments than tumor cells, and highest toxicity was found at selleck inhibitor the highest dosage. Likewise in Table 2 the interaction of both drugs at different concentrations are shown. We found a syner gistic effect in HeLa and SiHa cells with the two lowest doses. With the dose of PTX 8 mM CIS 4 uM, the drugs interaction can be considered as nearly additive. Finally with highest dose we found a different behavior, so that in HeLa cells we observed a clear antagonic effect. However at highest dose in SiHa cells it was observed a synergic effect. In non tumorigenic HaCaT cells with highest dose showed a synergic effect and with other doses a nearly additive effect was found.

Survival Cells Inhibitors,Modulators,Libraries in vitro The survival index was determined by WST 1 assay and we found 63. 62. 1% and 57. 81. 0% in HeLa and SiHa cells exclusively treated with PTX respectively. Surprisingly, survival was higher in HeLa cells and SiHa cells treated with CIS, than in the groups treated solely with PTX. The most important toxic effect was observed in PTX CIS groups. Cell survival after treatment was 40. 21. 0% in HeLa and 33. 01. 2% in SiHa cells. In contraposition, the addition of CIS to non tumor HaCaT cells exhibited practically no effect on their survival rate, and the PTX or PTX CIS treat ments slightly decreased the surviving cells. These data demonstrate Inhibitors,Modulators,Libraries that PTX per se possesses toxic properties and produces a significant increase of CIS cytotoxicity in human HeLa and SiHa cervical cancer cell lines.

Early detection of apoptosis in cervix cancer cells Early stage of apoptosis was detected by flow cytometry using annexin V and apoptosis progresses by DNA frag ments Enzyme linked immunosorbent assay. Table 3 displays results for both tests. All groups showed excellent correlation between both tests. We then observed the same behaviour as in preceding experiment, higher toxicity with Inhibitors,Modulators,Libraries the drug combination in comparison with PTX, CIS Inhibitors,Modulators,Libraries or control treatments in SiHa cells P 0. 001. PTX induces early apoptosis in HeLa cells. In contrast, PTX alone in non tumor HaCaT cells did not induce early apoptosis in these cells. The most important induction of early apoptosis was observed only in the CIS treated group. Finally, HaCaT cells cultures in the presence of PTX CIS exhibited an early apoptosis level comprising that between CIS and untreated control cells.

PTX sensitizes cervical cancer cells to CIS induced late apoptosis induced through caspase activation Apoptosis can be reversible in the first Inhibitors,Modulators,Libraries steps. for this reason we also determined late apoptosis by epifluores cence. Figure 1A shows that in all cases in untreated control groups, the apoptotic sellectchem index was 13. In con trast, in all treated groups, important levels of apoptosis were detected, because when HeLa and SiHa tumor cells were treated with PTX alone, the apoptotic indexes were 43. 84.