A total of 2 MMT fe mice were weighed and randomly assigned to cages in the four  Genistein treatment arms as described above. The treatment groups consisted of: control and letrozole , , and mg/kg . Study Design The drugs were dissolved in DMSO and then diluted in peanut oil such that μl delivered the appropriate doses. The final concentration of DMSO in all solutions was . Control mice received a solution of peanut oil with DMSO. All mice were dosed daily by oral gavage using a stainless ste 0-gauge gavage needle . All mice were weighed and palpated for the presence of new mammary tumors once a week. When possib tumors were measured in three dimensions using calipe and an approximate volume was calculated using the formula height of the tumor.

Once the tumor reached the cumulative volume of cm , or was abscessed or visibly  Baicalein 491-67-8 impairing the mobility of the mou the mice were euthanized by CO asphyxiation. Whole blood was collected by cardiac puncture and placed in heparinized tubes. The blood was centrifuged to collect plasma for HPLC analysis. Following blood collecti the following tissues were harvested for HPLC analysis: liv tum and spleen. Mice were gavaged daily with tamoxif letrozole or control. Tumor incidence and histology were thenpared among Downloaded from clincancerres.aacrjournals on March 9, Copyright American Association for Cancer Research Author Manuscript Published OnlineFirst on March 0. DOI: .CCR Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. treatment groups. The estrogen receptor status of the tumors was determin as SERMs and aromatase inhibitors have only been shown to be effective in ER-positive tumors. Tissue and plasma concentrations of tamoxifen and buy Paeonol letrozole were assessed using high performance liquid chromatography .

The lowest dose of each agent that provided a statistically significant survival advantagepared to control was used in later studies. Immune Phenotype in Immunized Fe MMT Mice. This study was designed to examine the impact of immunization on T or T  MK-8669 AP23573 cytokine polarization in fe MMT mice. A total of 6 fe MMT mice were divided into four treatment groups: untreat cyclophosphamide on placebo and vaccine . To reduce T-suppressor lymphocyte activi all mice except those in the untreated group received a single intraperitoneal injection of cyclophosphamide three days before beginning the L-BL 5 vaccine regimen . Mice in the vaccine group were then subcutaneously injected with gauge needle once each week for eight weekslacebo mice were injected with μl of the empty liposomes. Following the and th dose of vaccine or place blood was collected via submandibular bleeds. Blood was pooled within a treatment group and serum was isolated for cytokine analysis.

Effects of Hormonal Therapy on L-BL 5 Immune Response. A total of 0 MMT fe mice were weighed and assigned to cages. On study day , mice were assigned to four treatment  amines arms with approximately equal average weights and tumor volumes: contr tamoxifen 0 mg/ letrozole mg/kg and estradiol mg/kg . Control mice received peanut oil containing DMSO. Downloaded from clincancerres.aacrjournals on March 9, Copyright American Association.