Interest in niche comparisons, as well as shifting biogeographic ranges, are therefore relevant to understanding the effects of climate change. Differential utilization of habitat by the two related species enables us to

test for concomitant differences in the molecular mechanisms that respond to a variety of stressors, particularly thermal stress. An important expression response to thermal stress is up-regulation of genes encoding heat-shock proteins (HSPs). HSPs promote cellular thermal tolerance through Doxorubicin molecular weight a variety of mechanisms, including protein folding or chaperoning of existing and newly synthesized proteins, aggregation suppression, reactivation of denatured proteins, shuttling proteins between different cell compartments, and destruction of damaged proteins (Vierling, 1991, Wahid et al., 2007 and Kotak et al., 2007). Though HSP induction is a universal response to heat-stress (Vierling, 1991), species from different climatic zones show different

HSP induction thresholds (Feder and Hofmann, 1999). Some of the most extreme examples come from Antarctic algae that induce HSPs at 5 °C (Vayda and Yuan, 1994), while hyperthermophilic Archaea require temperatures of 100 °C for HSP induction (Feder and Hofmann, 1999). In addition, the correlation between habitat temperatures and HSP induction thresholds has been observed

for congeners from habitats with much more subtle temperature differences (Ulmasov et al., 1992, Feder and Hofmann, 1999 and Knight, 2010). Ganetespib nmr Heat-stress tolerance is, however, a multigenic trait and non-HSP genes are also essential (Larkindale et al., 2005, Wahid et al., 2007 and Kotak et al., 2007). These include expression changes to allow the maintenance of membrane stability, scavenging of reactive oxygen species, production of antioxidants, accumulation and adjustment of compatible osmolytes and induction of signaling cascades (Wahid et al., 2007 and Kotak et al., 2007). It has further been suggested that the acute stress response and the long term adaptation to stress are Dichloromethane dehalogenase based on separate mechanisms and that HSP expression does not necessarily play a major role for the evolutionary adaptation to higher temperatures (Sørensen et al., 2007). In this study we use RNA-seq to investigate the inter-specific transcriptomic response of Z. marina and N. noltii under a simulated heat wave based on actual conditions that occurred in the southwestern Baltic Sea in 2003, in which Z. marina populations were decimated ( Reusch et al., 2005). Expression profiles were investigated in a common-stress-garden design using plants from northern and southern European localities, where the species co-occur.