It is known that influenza viruses isolated and propagated in mammalian cells often remain genetically and antigenically closely related to the virus present in clinical specimens [26], [27] and [28]. Isolation in embryonated hens’ eggs and also in cells can lead to amino acid changes in the hemagglutinin, which can occasionally alter antigenicity rendering the isolates unsuitable as candidate vaccine viruses [29], [30] and [31]. Cell culture isolates may thus increase the number of viruses available for vaccine virus selection and regulatory authorities are willing consider such viruses for the production

of influenza vaccines [24] and [32]. In the present study we evaluated the performance of vaccine manufacturing cell lines [12], [14], [15], [17], [33] and [34] for Selleckchem Bortezomib primary virus isolation from clinical specimens and analyzed the antigenic stability and antigen yields of resulting isolates in pilot-scale manufacturing processes. This

study was designed to serve two purposes. Cell lines used by vaccine manufacturers were evaluated for their permissiveness to isolate influenza viruses from clinical specimens. Genetic and antigenic stability, as well as the growth-characteristics of the isolates, were monitored Selleck DAPT in the homologous cell line and in those used by other manufacturers. Fig. 1 shows the 4 main experimental steps and the 3 critical performance parameters of this study. Twenty influenza virus-positive respiratory samples from patients with influenza-like Terminal deoxynucleotidyl transferase illness were included. These samples were collected in the USA or in Finland during the 2007–2008 and 2008–2009 influenza seasons. Four groups of five specimens were selected to represent each of the seasonal influenza subtypes: A(H1N1) viruses, A(H3N2) viruses,

influenza B viruses representing the Yamagata lineage and the Victoria lineage. Each original specimen was divided into 10 aliquots and stored at −80 °C until used for further experiments. Three different Madin-Darby canine kidney cell lines (MDCK-1[14] and [15]; MDCK-2[12], [14] and [33]; MDCK-3[33]) and one African green monkey cell line (VERO [17]) were used in the experiments. The MDCK-1 and MDCK-2 as well as the VERO cell lines were anchorage-dependent; whereas the MDCK-3 line was cultivated in suspension. The three MDCK cell lines were used for primary isolation of influenza viruses from clinical specimens and for pilot-scale virus production. The VERO cell line was used for small-scale production experiments, one representative isolate from each of the four virus groups (H1N1, H3N2, B-Victoria, B-Yamagata) was used. For production, MDCK-1 was grown on micro-carriers in serum free medium to which a protease was added to facilitate virus replication. Virus was harvested when cytopathic effect (CPE) was observed in all cells.