It DAPT clinical trial has been confirmed that children born prematurely constitute an at‐risk group, and may benefit from effective educational or therapeutic intervention programs. It is noteworthy that there is better knowledge about the evolution of preterm newborns who have evident neurological signs and receive early diagnosis; however, children with mild sequelae, which will manifest later,

are not yet provided proper attention, especially in the public healthcare system. Objective data documenting functional sequelae in these children may encourage the development of preventive programs for developmental stimulation, which are still scarce in Brazil. The authors declare no conflicts of interest. “
“Breast milk is the ideal food to newborns born at term and preterm, facilitating cognitive development.1 and 2 At a gestational age of less than 34 weeks, newborns are still unable to suck, swallow, and breathe

properly and coordinately. http://www.selleckchem.com/products/Dasatinib.html In such cases, the oral diet is administered through a feeding tube, which implies collecting, handling, storing, and administering human milk.3 These procedures may compromise the nutritional quality of breast milk, depriving preterm infants from a significant portion of calories from fat.4, 5 and 6 Vieira et al.7 observed a significant reduction in fat between natural donated breast milk (raw) milk and the milk that is offered. Among the processes related to supply of human milk studied, the greatest reduction occurred after the simulation of milk supply by continuous infusion. The process of freezing and thawing can change the physicochemical properties of breast milk and, therefore, the losses during continuous infusion Glutamate dehydrogenase could be affected by these changes. The freezing and thawing processes favor the formation of micelles, which can adhere to plastic, facilitating the loss of fat.4 and 7 Therefore, it became necessary to clarify whether this increased loss with continuous infusion might be caused by the thawing process or whether the

administration route (gavage or continuous infusion) would be the main responsible factor. The aim of this study was to analyze changes in the following macronutrients: fat, protein, and lactose in natural human milk, frozen and thawed, after administration simulation by gavage and continuous infusion. An experimental study was conducted with human milk samples from volunteer donors of the Human Milk Bank of the Instituto Nacional em Saúde da Mulher, da Criança e do Adolescente Fernandes Figueira, Rio de Janeiro, RJ, Brazil. All donors were mothers of newborns born at term, and the milk was collected in the morning. The milk was extracted by manual expression or electric pump and stored in glass vials.