RNA extraction and semi quantitative RT PCR The procedures for RNA extraction and semi quantitative reverse transcription polymerization chain reaction have been described previously. The primers used The correct sequences for all genes were confirmed by alignment with the reported sequence for each gene. A rat model of myocardial ischemia involving such information LAD ligation Male Sprague Dawley rats, aged 8 9 weeks, were randomly divided into three groups a sham control group, a group that underwent LAD ligation without GTP supplementation, and a group that underwent LAD ligation with GTP supplementation for 2 weeks, with 5 animals per group. Once Inhibitors,Modulators,Libraries the rat was anesthetized, the heart was exposed via a left Inhibitors,Modulators,Libraries thoracotomy, and a 6 0 polypropylene suture was tied onto the LAD coronary artery 3 mm distal to the inferior margin of the left atrium, and the chest wall was closed in layers.

Regional myocardial ischemia was confirmed by the observation of a rapid change from reddish to a dark red color on the anter ior surface of the LV and rapid development of akinesia and dilatation in the ligated area. All experimental procedures conformed to the Guidelines for Proper Inhibitors,Modulators,Libraries Conduct of Animal Experiments approved by the Animal Care and Use Committee of Taichung Veterans General Hospital and National Chung Hsing University. After surgery, the rats were fed intragastrically with GTPs every day for two weeks. After the rats were sacrificed, the hearts were cut along the long cross sectional axis of the left ventricle, and cardiac tissues at both the infarcted area and a remote site of myocardium were isolated to determine protein levels of the 67 kD laminin receptor and Cav 1 and 3 by immuno blotting, as described above.

Statistical analysis Quantitative values are presented as the mean and standard error of the mean. A difference was con sidered to be statistically significant when the Inhibitors,Modulators,Libraries P value was less than 0. 05. Results EGCg cardioprotective effects on cell viability, ROS formation, and cytosolic Ca2 overload in H2O2 treated H9c2 cells Previously, we have demonstrated that pre treatment with green tea extract protects cardiomyocytes from regional myocardial ischemia by overcoming cytosolic Ca2 over load, myofibril disruption, and alterations in adherens and gap junction protein levels and distribution in rats.

In the present study, we used a cell model of H9c2 rat cardiomyoblast to verify the cardioprotection of EGCg against the H2O2 induced oxidative stress during myocar dial Inhibitors,Modulators,Libraries ischemia assault. When exposed to 400 uM H2O2, an increase in oxidative stress caused morphological changes selleck chemical Idelalisib in H9c2 cells, which were accompanied by an increase in cell death that was prevented by 20 uM EGCg pre treatment for 30 min. Note that EGCg alone did not significantly alter cell morphology. In addition, the MTT assay showed a dose dependent decrease in cell viability of H9c2 cells treated with H2O2 from 100 to 400 uM.