The bar chart showed average weight

of rats per group at days 0, 7, 14 and 21 of sub-acute toxicity study. There is an obvious increase in the animal’s weight; it is shown to be continuous in the four treatment groups as well as the vehicle control. Zinc-aluminium levodopa nanocomposite high dose (ZALH 500 mg/kg), zinc-aluminium levodopa nanocomposite low dose (ZALL 5 mg/kg), zinc-aluminium nanocomposite high dose (ZAH 500 mg/kg), zinc-aluminium nanocomposite low dose (ZAL 5 mg/kg), vehicle control (VC 3-deazaneplanocin A concentration normal saline 100 ml/kg body weight). There is statistically significant difference (#) between day 0 and all other days in all the groups (p < 0.05). One-way ANOVA was used, and data are expressed BYL719 as means ± SD. Table 3 Coefficients of the brain, liver, spleen, heart and kidney Groups

Body weight (g) Brain (mg/g) Liver (mg/g) Heart (mg/g) Spleen (mg/g) Kidney (mg/g) ZALH (n = 8) 300 ± 25 5.61 ± 0.93 35.67 ± 1.53 4.00 ± 0.53 1.99 ± 0.37 4.19 ± 0.20 ZALL (n = 8) 342 ± 30 5.76 ± 0.55 36.27 ± 3.35 AZD5153 datasheet 3.90 ± 0.53 2.08 ± 0.20 4.16 ± 0.22 ZAH (n = 8) 337 ± 25 5.62 ± 0.31 30.14 ± 3.54 3.91 ± 0 .43 2.32 ± 0.26 3.98 ± 0. 23 ZAL (n = 8) 335 ± 47 5.22 ± 0.68 31.83 ± 4.12 4.50 ± 0.44 2.29 ± 0.19 3.93 ± 0.45 VC (n = 8) 332 ± 14 5.31 ± 0.70 28.25 ± 2.71 3.86 ± 0 .35 1.88 ± 0.19 3.59 ± 0.39 Mean coefficient of brain, liver, spleen, heart and kidney of all the groups. The coefficients of organs from the four treated groups were almost similar to those of the control. Statistical test used to compare the means of each group against the control group was done using one-way ANOVA; it shows no significant difference

with p > 0.05. Zinc-aluminium levodopa nanocomposite high dose (ZALH 500 mg/kg), zinc-aluminium levodopa nanocomposite low dose (ZALL Janus kinase (JAK) 5 mg/kg), zinc-aluminium nanocomposite high dose (ZAH 500 mg/kg), zinc-aluminium nanocomposite low dose (ZAL 5 mg/kg), vehicle control (VC normal saline 100 ml/kg body weight). Repeated doses or sub-acute toxicity study is aiming at evaluating target organ toxicity relative to cumulative exposure [9]. These kinds of studies are to be conducted at any point from initial discovery through to late-stage development of drugs and other substance including nanoparticle before clinical trial and human exposure [9]. These studies are conducted to detect potential hazards and assess risk in drug discovery. Aluminium and zinc are the two metals used in the synthesis of this delivery system. Zinc is considered a trace element with multiple beneficial effects especially in the immune system, phagocytosis, intracellular killing and cytokine production by the immune cells [10]. It may also act as an excellent antioxidant, with membrane stabilization ability, preventing free-radical-induced cellular injury [10].