The experiments were prepared in
triplicate and repeated three times. Bacterial cultures were centrifuged at 3000 g for 5 min at 4 °C to pellet the cells. The supernatants were removed and the bacterial cells were washed with sterile water three times. Then the cells were resuspended in PDB dilutions (PDB 1 : 50) to yield different working concentrations (0.33, 1, 1.67, 2.33, 3.0 and 3.67 × 107 CFU mL−1, using dilution plating on nutrient agar). These solutions were used to bioassay for trap formation. The negative controls were PDB dilutions (PDB 1 : 50). The experiments were prepared in triplicate and repeated three times. Bacteria learn more cells were obtained as described above and resuspended in PDB dilutions (1 : 50) containing 20% v/v bacterial cell-free
culture filtrate to yield different working concentrations (0.33, 1, 1.67, 2.33, 3.0 and 3.67 × 107 CFU mL−1). These solutions were used to bioassay for trap formation. The negative controls were 20% NB (v/v) prepared by PDB dilutions (PDB 1 : 50). The experiments were prepared in triplicate selleck inhibitor and repeated three times. Bacterial cells were resuspended to yield working concentrations of 1.67 × 107 CFU mL−1 in PDB dilutions (1 : 50) containing 5%, 10%, 20%, 30% or 40% v/v bacterial cell-free culture filtrates. These solutions were used to assay trap formation. Four control plates were included in each test run. Two control plates were PDB dilutions (PDB 1 : 50) containing 5% NB (v/v) without or with bacterial cells (final concentration, 1.67 × 107 CFU mL−1). And the other two were PDB dilutions (PDB 1 : 50) containing 40% NB (v/v) without or with bacterial cells (final concentration, 1.67 × 107 CFU mL−1). The experiments were prepared in triplicate and repeated three times. Arthrobotrys oligospora conidia were cocultivated with bacterial cells (final concentration, 1.67 × 107 CFU mL−1) containing or not containing 20%
bacterial cell-free culture filtrates in PDB dilution (PDB old 1 : 50). Arthrobotrys oligospora conidia were cocultivated (PDB 1 : 50) with bacterial cells (final concentration, 1.67 × 107 CFU mL−1) in sterile water. Negative controls were PDB dilution (PDB 1 : 50) containing 20% NB (v/v) or sterile water. Fungal pellets absorbed by variable volume pipette were placed into another Petri plate and washed with 10 mL sterile water three times, mounted on a stub and coated with gold. The specimens were examined using a FEI Quanta 200 scanning electron microscope from FEI Company (Hillsboro) in the high-vacuum mode at 20 kV. Each treatment was performed in duplicate and the experiment was repeated three times. Bacterial cells were resuspended to a working concentration of 1.67 × 107 CFU mL−1 prepared by sterile water or a nutrient solution consisting of PDB, a series of dilutions of PDB (1 : 5, 1 : 10, 1 : 20, 1 : 30, 1 : 50, 1 : 100, 1 : 200) containing 20% v/v bacterial cell-free culture filtrates. These solutions were used to assay trap formation.