The IR spectrum affirmed the sulfonyl group at 1365 cm−1 and –NH– group at 3203 cm−1. In aromatic section of 1H NMR spectrum, the signals of p-substituted GW 572016 phenyl ring linked to sulfonyl group appeared as two doublets integrated for two protons each with coupling constant of 8.4 Hz, one at δ 7.69

(ortho to the sulfonyl group) while other at δ 7.42 (meta to the sulfonyl group). The signals appearing at δ 7.52 (d, J = 2.4 Hz, 1H, H-6), 6.96 (dd, J = 8.8, 2.4 Hz, 1H, H-4) and 6.63 (d, J = 8.8 Hz, 1H, H-3) were allotted to three protons of tri-substituted aniline ring. In the aliphatic section of 1H NMR spectrum, the signals revealed at δ 3.62 (s, 3H, CH3O-2) for methoxy group at 2nd position of substituted aniline & 1.28 (s, 9H, (CH3)3C-4′) for tertiary butyl group at 4th position of other benzene ring. Thus the structure of compound (3a) was corroborated and named as N-(5-Chloro-2-methoxyphenyl)-4-ter-butylbenzenesulfonamide. The mass fragmentation pattern of 3a is clearly sketched in Fig. 1. Similarly, the structures see more of other synthesized compounds were characterized by 1H NMR, IR and EI-MS as described in experimental section. The results of % age inhibition & MIC values for antibacterial activity of the synthesized compounds against Gram-negative & Gram-positive bacteria are described in Table 1. The compounds N-(5-Chloro-2-methoxyphenyl)-N-ethyl-4-ter-butylbenzenesulfonamide

(6a) expressed activity against all the bacterial strains with good % age inhibition & MIC values relative to the reference standard ciprofloxacin, probably due to presence of N-substitution of ethyl and ter-butyl groups in the molecule. The compounds 3b, 3c, 3e, 6a, 7d & 7e were active against the both bacterial strains of Gram-positive. The compounds 6b, 6c, 6d, 6e, 7a & 7c were inactive against all the bacterial strains of Gram-negative & Gram-positive bacteria. These compounds can further be exploited and their derivatives could be synthesized to get MIC values near to standard. So these compounds might be potential target in the drug discovery Casein kinase 1 and development programme. The synthesized compounds are well

supported by spectroscopic data. From the antibacterial activity data (Table 1), it is concluded that the series of compounds depicted remarkable inhibitory action against different bacterial strains. Synthesis, biological activity evaluation and estimation of SAR of some more analogues are under investigation. In this way, the compounds could be potential target in the discovery of medicine and drug development programme. All authors have none to declare. “
“Cancer is one of the most dangerous diseases in humans and presently there is a considerable scientific discovery of new anticancer agents from natural products.1 Natural product-based medicines, particularly, herbal- based drugs represented about 60–80 percent of all drugs in use by 1990.