MV at a movement of the activation curve to more negative potentials. BIBR 1532 Modification of a curve of steady state inactivation was not detected, indicating that the L-type calcium-channels The ventricular Ren myocytes were probably activated by acute stress. The results showed that the regulation of ICa L with various Nderten channel activation dependent-Dependent phosphorylation is connected. Au Addition marked the activity t of protein kinase A increased Hte fa Successively acute stress, L and ICa inhibited by a protein kinase inhibitor, suggesting that acute stressinduced The increase in ICa implies activation of PKA. Expression of L-type calcium channel subunit by improved 1c chronic stress, the purpose of this experiment was to determine whether the expression of L-type calcium channel subunit were 1c of chronic stress regulated and whether these Amending Regulation instead of L ICa in ventricular Ren myocytes in chronic stress zusammenh depends.
Semi-quantitative RT-PCR analysis was first used to test the hypothesis. As shown in FIG. 4A is the 1c subunit of L-type calcium channel blocker was expressed in the two groups, and have a h Here expressed in ventricular Ren Myocardium in rats exposed to chronic stress than the BMS-582664 control. Additionally Tzlich Northern blot analysis was performed to assess the effect of chronic stress on mRNA levels. According to obtain RT-PCR findings, the results of the Northern blot showed that the mRNA of the 1c subunit of the L-type calcium channel blockers in chronic stress is more abundant and significantly increased Ht was about 1.
6 times compared to the control In addition, immunoblot analysis also showed that the amount of protein subunit 1c in the heart of stressed rats increased ht, about 17% more than it embroidered. Our results with previous work on the L-type calcium channel, suggest that chronic stress on regulation of expression subunit lead 1c, and it can be a great s molecular mechanism of Erh Increase ICa L be induced by chronic stress. The channel voltage-gated L-type calcium plays an r Essential role in regulating a variety of cellular Ren processes, including normal membrane excitability, Ca2 homeostasis Hom, Protein phosphorylation and gene regulation. In the heart of h Excitation contraction coupling depends on L-type calcium channel calcium channel LTYPE heart membrane is a multimer Se subunit 1c formation of pores and Regulations 2 / ? and subunits assembled.
Subunit contains lt The voltage sensor and 1c receptors for various classes of calcium antagonists / agonists, and determines the basic electrophysiological properties. In this study, we have that chronic stress increased ICa L represented density and increased Not hte ICa L accompanied by a deterioration in the properties of activation and inactivation, but corresponded to the regulatory levels of mRNA and protein L-type calcium channel subunit 1c.