These miRNAs may be useful for diagnosing biliary cancer and determining Temsirolimus clinical trial its prognosis. Disclosures: The following people have nothing to disclose: Hiizu Fujihara, Masao Honda, Hikari Okada, Takashi Kagaya, Hajime Takatori, Mikiko Nakamura Changes in DNA methylation patterns are believed to be an early event in hepatocarcinogenesis. The aim of our study is to analyze the methylation frequency of tumor suppressor genes; P14, P15, P73 and Mismatch repair gene (O6MGMT) in HCV related chronic liver disease and HCC to identify candidate epigenetic biomarkers for HCC predication. Methods: 516 Egyptian patients with HCV-related liver disease were recruited from Kasr Alaini multidisciplinary HCC clinic from April 2010 to January

2012. Subjects were divided into 4 different clinically defined groups; HCC group (n=208), liver cirrhosis group (n=108), chronic hepatitis C group (n=100), and Control group (n=100); to analyze the methylation status of tumor suppressor genes; p14, P15, P73 and the DNA Mismatch repair gene (〇6MGMT) in patients’ plasma by using EpiTect Methyl qPCR Array technology. Methylation frequency was considered to be hypermethylated

if >10% and/or intermediately methylated if >60%. Result: In our series, a significant difference in the hypermethylation status of all studied genes was noted within the different stages of chronic liver disease and ultimately HCC. Hypermethylation of the P14 gene was detected in 100/208 (48. 1%), 52/108 (48. 1%), 16/100 (16%) and 8/100 (8%) among HCC, liver cirrhosis, chronic hepatitis and control groups respectively with a statistically significant difference BAY 57-1293 in vivo between the studied groups; (p=0. 008). We also reported P15 hypermethylation in 92/208 (44. 2%), 36/108 (33. 3%), 20/100 (20%) and 4/100 (4%) among HCC, liver cirrhosis, chronic hepatitis and control groups respectively with a statistically significant difference between the studied groups; (p=0. 00o). In addition, more hypermethylation frequency of P73 was detected in 136/208 (65. 4%), 72/108 (66. 7%), 32/100 (32%)

and 4/100 (4%) among HCC, liver cirrhosis, chronic hepatitis and control groups respectively with a statisti-cally significant difference between the studied groups; (p<0. 001). Also, we detected 〇6MGMT hypermethylation in 84/208 (40. 4%), 60/108 (55. 3%), 20/100 (20%) and 4/100 (4%) 上海皓元 among HCC, liver cirrhosis, chronic hepatitis and control groups respectively with a statistically significant difference between the studied groups; (p<0. 001). Conclusion: The epigenetic changes observed in this study shows that HCC tumors exhibit specific DNA methylation signatures associated with the potential clinical applications in diagnosis and prognosis. On the other hand, methylation frequency could be used to monitor whether the patient with chronic hepatitis C will be subjected to liver cirrhosis or even HCC. So, we can conclude that methylation process in an early event in hepatocarcinogenesis.