You anesthetic under light. The Group Wnt Pathway controls If the suspension of celecoxib in f Ssriger L Solution, which administered 0.5% carboxymethylcellulose. The left eye of the animal was kept untreated. at the end of 15 minutes, the animals were euthanized by intraperitoneal injection of sodium pentobarbital. The eyes were enucleated immediately after euthanasia and snap-frozen using a dry ice isopentane bath. Periokul Re tissues were collected from both eyes.

Wnt Pathway chemical structure

The eyes were dissected while frozen with ceramic tile and dry ice bath, while avoiding cross-contamination of tissues may need during the dissection. Samples isolated from ocular tissues were stored at 0 to for further processing. Drug levels in the sclera, RPE choro The retina, Glask Body, lens and periocular tissues were MS by LC S /. Treatment of samples. The concentrations of celecoxib and its precursors in the fields of transport, were the binding of melanin and metabolic stability of t studies analyzed by simple dilution of samples of the study with acetonitrile to reduce the salt concentration. Drug levels in samples of ocular tissues in the rat, by extracting the drug from samples of ocular tissue by the method of Proteinf Filling acetonitrile protected shops.
Briefly, samples of eye tissues were treated with 250 l of PBS was mixed for containingorder% cumulative transport across bovine CSA SCRPE CMA Celecoxib. The transport through SCRPE% cumulative time was 8.0 h Higher than the AUC for celecoxib and traffic was markedly Ago as the CMA and CAA. Based on these results, CSA was selected for further investigation of selected prodrug. CSA prodrug bioconversion in vitro. In vitro metabolic stability of t and biotransformation of CSA were evaluated in ocular tissues in rats and SD-plasma samples. In vitro incubation of fresh rat ocular tissues with CSA and plasma samples showed 10 conversion 0% of the AUC of celecoxib in 30 min. If the formation of celecoxib was tissue mass, EPR choro The normal retina and showed the maximum conversion of CSA to celecoxib, w While glass lens, plasma and tissue around the eyes showed any conversion. To the metabolic enzymes in the bioconversion of the AUC of celecoxib in vitro assays were involved bioconversion performed in the presence of enzyme inhibitors. As shown in Figure 5A, the formation of celecoxib inhibited the metabolism of CSA significantly in the presence of bis-phosphate, an inhibitor of the amidases in RPE choro Of the retina and plasma samples. Esterases has also significantly contributed to the transformation of celecoxib AUC in all tissue samples.
As shown in Figure 5B, the metabolic conversion of CSA was significantly inhibited in the presence of paraoxon, an inhibitor of esterases. In addition, cytochrome P 450 has also browse the metabolic conversion of CSA into the sclera, the choro Of RPE, and retina, as indicated by a significant inhibition of the metabolic conversion in the presence of CYP450 inhibitor 1 indicated aminobenzotriazole. In the presence of a mixture of all three inhibitors celecoxib formation was induced by 90% in all tissues, au It inhibits the periocular tissues. In vivo tissue distribution of CSA and celecoxib in pigmented rats. The tissue distribution of celecoxib and CSA in ocular tissues after subconjunctival injection after posterior pigmented rats shown in Figure 6. As shown in Figure.