D in a mass spectrometer equipped with an ion spray ionization source. A 1:1 GSK256066 mixture of water and methanol, 1% acetic acid, Facilitate the ionization was, as L Used solvents. Figure 3 shows the mass spectrum of a mixture of 10 M baicalein with 10 ML Solution of Fe2 or Fe3 fra YEARS Made Riger. In both cases The protonated ligand at m / z 271 was observed. 7 and the species corresponding to the formation of a complex between 2:01 baicalein and iron respectively observed: one baicalein2] Fe2 complex 3A was used when Fe2 and Fe3 complex baicalein2 2], if Fe3 3B was used. A close look at the model complex Fe isotope baicalein suggests that there is good distribution of iron isotopes. Formation of a complex is likely to Fe3 baicalein2 by the acidic conditions used in the study, ESI MS favors.
This observation was best by spectrophotometric titration Saturated with 10 M in 30 mM Fe3 baicalein NaAc buffer, pH 4 5th Third Third Measures the binding affinity of t Of iron with baicalein conditional binding constants with Fe2 and Fe3 baicalein in 20 mM KPB, pH investigated the seventh 2-298 K, as described in WZ8040 the experimental part. It has been protected by method 1 gesch Than apparent st Ndiger connection Complex 2 is Fe2 ? 1011 9 M 2 And apparent st Ndiger connection for the complex Fe3 baicalein is ? 3106 M 1, w While the corresponding values process are ? second February 1011 M 2 and M 1 ? 1106th The values obtained by both methods are in good agreement, and the differences are within a factor of about 4 Under Similar conditions, it seems that baicalein Fe2 size one Enordnung h Ago than quercetin Tats Chlich binds, suggesting that baicalein can be more effective than quercetin in Eisenhom Homeostasis modulation under physiological conditions.
Third 4th Competition for Fe2 between baicalein and constant liaison ferrozine above data indicate a strong connection between Fe2 and baicalein relevant physiological conditions. To this connection St rkeren competition experiments between baicalein and a well-known chelating Fe2 to check ferrozine third? 65 1015, in 20 mM buffer at 298 K were carried out 4, the KBP Ver Shows change in the absorption time of Fe2 ferrozine3 complex after addition of a st Stoichiometric amount of baicalein. W During the first hour, no Ver Change ferrozine for Fe peak was observed at 562 nm, but an increase in the intensity t 400 450 nm was observed, probably.
By the formation of a hybrid complex Fe2 baicalein ferrozine Iron complexes Similar hybrids with amino acids FerroZine and reported. After the first hour, a steady decrease in the absorption peak was coupled ferrozine3 Fe2 with a simultaneous increase in the region of 400 450 nm absorption was observed. This spectral Ver Change indicates that the formation of complex baicalein Fe2 co With the dissociation of the complex Fe2 ferrozine3 Combine to falls. This experiment clearly shows that baicalein strongly binds Fe2 relevant under physiological conditions and the connection is st Known stronger than Fe2 chelator ferrozine. This observation is calculated in accordance with the binding constants. Third 5th 1H NMR studies of metal binding studies, 1H NMR titration site were carried out to the probable site of iron binding identify bai