AIHA, autoimmune hemolytic anemia; AMA, anti-mitochondrial autoantibody; dnTGF-βRII, dominant-negative transforming growth factor-β receptor II; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; IL, interleukin; PBC,
primary biliary cirrhosis; PBMC, peripheral blood mononuclear cells; PCR, polymerase chain reaction; PDC-E2, pyruvate dehydrogenase complex, E2 component; RA, rheumatoid arthritis; SLE, systemic lupus erythematosus; TGF-β, transforming growth factor-β; Selleck Opaganib TNF-α, tumor necrosis factor-α; Treg, regulatory T cells; UDCA, ursodeoxycholic acid; WBC, white blood cell. Female patients between the ages of 18 and 65 years diagnosed with PBC based on the presence of an AMA titer > 1:40, alkaline phosphatase at least twice the upper limit of normal, and liver histology compatible with stage I-III PBC, and who did not have normalization of their alkaline phosphatase after a minimum of 6 months of treatment with adequate doses of UDCA, were enrolled. Patients were excluded if they had evidence of decompensated liver disease (ascites, jaundice, coagulopathy, hepatic encephalopathy, or varices), other coexisting liver disease, treatment with immunosuppressive medications Talazoparib molecular weight within 4 weeks of enrollment, or active infection. Permitted medications included prednisone of 10 mg daily or less and UDCA at a dose that was maintained at pre-enrollment doses. This was an open-label study conducted at
a single academic clinical research center (ClinicalTrials.gov, Identifier: NCT00364819). After a screening visit, all subjects were treated with rituximab 1000 mg by intravenous infusion on days 1 and 15. Before rituximab infusion, patients received 100 mg of methylprednisolone intravenously. Safety assessments included a clinic visit and laboratory tests performed on the days of infusion as well as at 4, 8, 16, 24, 36, and 52 weeks as well as
a liver biopsy at 52 weeks. Blood was also collected at each visit for B-cell MCE公司 and T-cell functional assays. In addition, the PBC-40 questionnaire, a validated tool for the assessment of quality of life in patients with PBC25 was administered before treatment and at week 52. The study was initially planned to enroll 10 patients but was closed after six patients due to low enrollment. During the enrollment period, 24 patients with PBC and an incomplete response to UDCA were screened. Three subjects had cirrhosis and 15 subjects declined to participate. The study was approved by the Institutional Review Board, and all subjects gave written informed consent before enrollment. Peripheral blood mononuclear cells (PBMCs) were isolated by density gradient using Histopaque-1077 (Sigma Chemical Co., St. Louis, MO), and the cells were washed and resuspended in phosphate-buffered saline (PBS) (Mediatech Inc., Herndon, VA) containing 0.5% bovine serum albumin (BSA; Fraction V, OmniPur; EMD Chemicals Inc., Gibbstown, NJ) and 0.05% EDTA (Sigma Chemical Co.).