Preconditioning of both donors and recipients with DS significant

Preconditioning of both donors and recipients with DS significantly reduces IRI and thus improves graft function after eKTx.”
“SETTING: The uptake of tests endorsed by the World Health Organization to detect and appropriately confirm multidrug-resistant tuberculosis (MDR-TB) in low-income countries remains insufficient.

OBJECTIVE: To validate the implementation of line-probe assays (LPA) and liquid culture to develop an algorithm to detect MDR-TB in the challenging setting of Haiti.

METHODS: Through an EXPAND-TB (Expanding Access to New Diagnostics for TB) partnership, proficiency testing and

validation of 221 acid-fast bacilli positive specimens were performed. Sensitivity, cost and processing time were analysed.


Volasertib Using liquid vs. solid culture shortened the turnaround time from 54 to 19 days, with a sensitivity of 100% vs. 98.6% and a total cost reduction of 13%. LPA detected all TB and MDR-TB cases at a lower cost than culture, in a mean time of 7.5 days.

CONCLUSION: The combined use of molecular and liquid culture techniques accelerates the accurate diagnosis of TB and susceptibility testing against first-line drugs in a significantly shorter time, and is less expensive. The implementation of this new algorithm could significantly and accurately improve the screening and treatment follow-up of patients affected with TB and MDR-TB.”
“Application of a sensitive and rapid flow injection analysis (FIA) method for determination of topiramate, piracetam, and levetiracetam Z-DEVD-FMK inhibitor in pharmaceutical formulations Smoothened Agonist in vivo has been investigated. The method is based on the reaction with ortho-phtalaldehyde and 2-mercaptoethanol in a basic buffer and measurement of absorbance at 295 nm under flow conditions. Variables affecting the determination such as sample injection volume, pH, ionic strength, reagent concentrations, flow rate of reagent and other FIA parameters were optimized to produce the most sensitive and reproducible results using a quarter-fraction factorial design, for five factors at two levels. Also,

the method has been optimized and fully validated in terms of linearity and range, limit of detection and quantitation, precision, selectivity and accuracy. The method was successfully applied to the analysis of pharmaceutical preparations.”
“Thylakoid membranes have a unique complement of proteins, most of which are nuclear encoded synthesized in the cytosol, imported into the stroma and translocated into thylakoid membranes by specific thylakoid translocases. Known thylakoid translocases contain core multi-spanning, membrane-integrated subunits that are also nuclear-encoded and imported into chloroplasts before being integrated into thylakoid membranes. Thylakoid translocases play a central role in determining the composition of thylakoids, yet the manner by which the core translocase subunits are integrated into the membrane is not known.

This article describes those domains and offers suggestions of sp

This article describes those domains and offers suggestions of specific instruments to use in clinical or research settings.”
“Imaging, clinical, and pre-clinical studies have provided ample evidence for a cerebellar involvement in cognitive brain function including cognitive brain disorders, such as autism and schizophrenia. We previously reported that cerebellar activity modulates dopamine release in the mouse medial

prefrontal cortex selleckchem (mPFC) via two distinct pathways: (1) cerebellum to mPFC via dopaminergic projections from the ventral tegmental area (VTA) and (2) cerebellum to mPFC via glutamatergic projections from the mediodorsal and ventrolateral thalamus (ThN md and vl). The present study compared functional adaptations of cerebello-cortical circuitry following developmental cerebellar pathology in a mouse model of developmental loss of Purkinje cells (Lurcher) and a mouse model of fragile X syndrome (Fmr1 KO mice). Fixed potential amperometry was used to measure mPFC dopamine release in response to cerebellar electrical stimulation. Mutant mice of both strains showed an attenuation in cerebellar-evoked mPFC dopamine release compared to respective wildtype mice. This was accompanied by a functional reorganization of the VTA and thalamic pathways mediating cerebellar modulation of mPFC dopamine release. Inactivation of the VTA pathway by intra-VTA lidocaine or kynurenate infusions

decreased dopamine release by 50 % in wildtype and 20-30 % in mutant mice of both strains. Intra-ThN vl infusions of either drug decreased dopamine release by 15 % in wildtype and 40 % in mutant mice of both strains, while dopamine release remained relatively unchanged QNZ manufacturer following intra-ThN md drug infusions. These results indicate a shift in strength towards the thalamic vl projection, away from the VTA. Thus, cerebellar neuropathologies associated with autism spectrum disorders may cause a reduction in cerebellar modulation of mPFC dopamine release that is related to a reorganization of the mediating neuronal pathways.”
“We evaluate the feasibility of manipulating droplets in two this website dimensions by exploiting Coulombic forces acting on

conductive droplets immersed in a dielectric fluid. When a droplet suspended in an immiscible fluid is located near an electrode under a dc voltage, the droplet can be charged by direct contact, by charge transfer along an electrically conducting path, or by both mechanisms. This process is called electrical charging of droplet (ECOD). This charged droplet may then be transported rapidly by exploiting Coulombic forces. We experimentally demonstrate electrical actuation of a charged droplet by applying voltage sequences. A charged droplet is two dimensionally actuated by following the direction of the electrical field signal. The droplet does not contact the surface of the microfluidic chip when it moves. This characteristic is very advantageous because treatments of the substrate surfaces of microfluidic chip become simpler.

Methods: Ninety-four term infants

delivered vaginally (gr

Methods: Ninety-four term infants

delivered vaginally (group 1, n = 31), cesarean section (C/S) with general anesthesia (GA) (group 2, n = 29), and C/S with epidural anesthesia (EA) (group 3, n = 34) were included in this study. All infants were weighed at birth and on the second day of life and intravenous (IV) fluid infused to the mothers for the last 6 h prior to delivery was recorded. Serum electrolytes, osmolality, N-terminal proANP (NT-proANP), brain natriuretic peptide (BNP), aldosterone and plasma antidiuretic RO5045337 hormone (ADH) concentrations were measured at cord blood and on the second day of life.

Results: Our research showed that postnatal weight loss of infants was higher in C/S than vaginal deliveries (5.7% vs. 1.3%) (p < 0.0001) and in EA group than GA group (6.8% vs. 4.3%) (p < 0.0001). Postnatal weight 4EGI-1 mw losses were correlated with IV fluid volume infused to the mothers for the last 6 h prior to delivery (R = 0.814, p = 0.000) and with serum

NT-proANP (R = 0.418, p = 0.000), BNP (R = 0.454, p = 0.000), and ADH (R = 0.509, p = 0.000) but not with aldosterone concentrations (p > 0.05).

Conclusion: Large amounts of IV fluid given to the mothers who were applied EA prior to the delivery affect their offsprings’ postnatal weight loss via certain vasoactive hormones.”
“QUESTIONS UNDER STUDY: recombinant activated factor VII (rFVIIa) is used off-label for massive bleeding. There is no convincing evidence of the benefits of this practice and the minimal effective dose is unknown. The aim of the study was to evaluate our in-house guideline recommending a low dose of 60 mu g/kg for off-label use of rFVIIa.

METHODS: observational cohort study at the Inselspital Bern, a tertiary care University Hospital in Switzerland. All patients with massive bleeding treated off-label with rFVIIa between January 2005 and December 2007 were included. Survival, change of bleeding and transfusion rates, coagulation parameters Torin 2 research buy and complications were analysed.


seventy-three patients received rFVIIa. Severe haemorrhage was documented by a bleeding rate of 1000 mL/h (median; interquartile range 350-3000) and total volume replacement of 11.9 L (6.6-15.2) before administration of rFVIIa. The median rFVIIa-dose was 64 mu g/kg (56-71). rFVIIa was administered once in 79% patients, twice in 18%. The bleeding rate was reduced in 82% of the patients. Transfused packed red blood cells decreased from 14 units (8-22) over 4.9 h (2.5-8.8) before rFVIIa to 2 (0-6) in 24 h thereafter, platelet concentrates from 2 units (1-3) to 1 (0-2) and FFP from 11 units (6-16) to 2 (0-9). In-hospital mortality was 14% within 24 h and 32% at day 30. There were two arterial thromboembolic complications possibly related to rFVIIa.

The average number of YPLL per death was approximately 15 for mel

The average number of YPLL per death was approximately 15 for melanoma and 10 for NMSC. We found the costs attributable to melanoma and NMSC ranged from SUS39.2 million to $US28.9 million for morbidity and

$US3.3 billion to $US1.0 billion for mortality, respectively.

It is clear from the published literature that skin cancer leads to significant YPLL and indirect costs associated with premature mortality and morbidity. Prevention and early detection efforts are important in helping reduce the incidence of melanoma and NMSC, and the related deaths and productivity losses.”
“The halogen-free flame retardant (HFFR) ethylene-vinyl acetate copolymer (EVM)/ATH/SiO2 composites have been prepared by melting compounding method, JQ1 price and the flame retardant, thermal stability, rheological, electrical, and mechanical properties have been investigated by cone calorimeter, LOI, UL-94, TG, FE-SEM, rotational rheometer, dielectric breakdown, and ultimate tensile. The results indicate that the flame retardant of EVM vulcanizates is improved and the fire jeopardizing is dramatically reduced due to the addition of ATH. It is necessary that sufficient loading of ATH (>= 120 phr) is needed to reach essential level (LOI > 30; V-0 rating) of flame retardant for HFFR EVM/ATH/SiO2 composites used as cable in industry. The rheological

characteristics show that at all the measurement GANT61 datasheet Ulixertinib price frequencies, the storage and loss modulus of the composites increase monotonously as the concentration of ATH filler increases, while the complex viscosity and tan delta present reverse trend. And also, it has been found that the HFFR composites at high filler concentrations still keep good mechanical and electrical properties, which is very important for practical applications as cable. (C) 2010 Wiley Periodicals, Inc. J Appl Polym Sci 120: 368-378, 2011″
“Bovine serum albumin-loaded beads were prepared by ionotropic gelation of alginate with calcium chloride and chitosan. The effect of sodium alginate concentration and chitosan concentration on the particle size and loading efficacy was studied. The diameter

of the beads formed is dependent on the size of the needle used. The optimum condition for preparation alginate-chitosan beads was alginate concentration of 3% and chitosan concentration of 0.25% at pH 5. The resulting bead formulation had a loading efficacy of 98.5% and average size of 1,501 mu m, and scanning electron microscopy images showed spherical and smooth particles. Chitosan concentration significantly influenced particle size and encapsulation efficiency of chitosan-alginate beads (p<0.05). Decreasing the alginate concentration resulted in an increased release of albumin in acidic media. The rapid dissolution of chitosan-alginate matrices in the higher pH resulted in burst release of protein drug.

Imaging studies included x-ray of the maxilla and chest, ultrasou

Imaging studies included x-ray of the maxilla and chest, ultrasound of the abdomen, computed tomography scan (1990 to 1999), and magnetic resonance imaging (after 1999). BV-6 All surgeries were performed using endotracheal anesthesia, and complete gross excision of the tumor was achieved with coverage of the defect with mucoperiosteal flaps. All specimens were subjected to histopathology and immunohistochemistry.

Results: The expansion of the alveolus produced by the tumor improved in 4 to 6 months. Subsequent dentition was affected by the removal of involved tooth buds during the operation.

All the patients are in regular follow-up (maximum 206 months)and there has been no local recurrence or distant metastasis. Mean follow-up time Was 130.8 months (95% confidence interval, 168.8-210.6). Overall survival at 17 years was 85.6%. Median survival could not be established clue to statistically insignificant sample size, while mean

survival time was 189.7 months (95% confidence interval, 103.7-157.8).

Conclusions: beta-catenin assay In the absence of metastatic disease, melanotic neuroectodermal tumors of infancy can be successfully managed by local excision. (c) 2009 American Association of Oral and Maxillofacial Surgeons”
“Studies of the effects of single-gene mutations on longevity in Caenorhabditis elegans, Drosophila melanogaster and Mus musculus identified homologous, highly conserved signalling pathways that influence ageing. In

each of these very distantly related species, single mutations which lead-directly or indirectly-to reduced insulin, insulin-like growth factor (IGF) or insulin/IGF-like signalling (IIS) can produce significant Selleckchem Ruboxistaurin increases in both average and maximal lifespan. In mice, most of the life-extending mutations described to date reduce somatotropic (growth hormone (GH) and IGF-1) signalling. The reported extensions of longevity are most robust in GH-deficient and GH-resistant mice, while suppression of somatotropic signalling ‘downstream’ of the GH receptor produces effects that are generally smaller and often limited to female animals. This could be due to GH influencing ageing by both IGF-1-mediated and IGF-1-independent mechanisms. In mutants that have been examined in some detail, increased longevity is associated with various indices of delayed ageing and extended ‘healthspan’. The mechanisms that probably underlie the extension of both lifespan and healthspan of these animals include increased stress resistance, improved antioxidant defences, alterations in insulin signalling (e.g. hypoinsulinaemia combined with improved insulin sensitivity in some mutants and insulin resistance in others), a shift from pro-to anti-inflammatory profile of circulating adipokines, reduced mammalian target of rapamycin-mediated translation and altered mitochondrial function including greater utilization of lipids when compared with carbohydrates.

001) than healthy controls However, only 12 9% of 124 GPCA-posit

001) than healthy controls. However, only 12.9% of 124 GPCA-positive patients were diagnosed as having PA by the WHO definition. Conclusion Only 12.9% of GPCA-positive patients are discovered to have PA by the WHO definition. Oral Diseases (2013) 19, 381-386″
“Palladium-catalyzed arylation of diamines of the adamantane series with isomeric 2-, 4-, and 6-chloroquinoline was studied, and optimal conditions for the synthesis of the corresponding N,N’-diaryl derivatives were found. N,N-Diarylation products of primary amino groups in the diamines bearing 2-aminoethyl and 4-aminophenyl substituents were readily formed.”
“Green synthesis of silver nanoparticles was carried out using Boerhaavia

diffuse plant extract as a reducing agent. The biosynthesized AgNPs were characterized by SEM-EDAX, XRD, TEM, this website UV-Vis, and FT-IR spectroscopy techniques. UV-Vis spectroscopy of prepared silver colloidal solution showed absorption maxima at 418 nm. XRD and TEM analysis revealed that AgNPs are face-centered, cubic structure being spherical in shape with an average particle size of 25 nm. The AgNPs were tested for antibacterial activity against three fish bacterial pathogens viz., Aeromonas hydrophila, Pseudomonas fluorescens and Flavobacterium branchiophilum and they demonstrated highest sensitivity toward F. branchiophilum when compared to other two bacterial pathogens. (C) 2013 Elsevier B.V.

All rights reserved.”
“Objective Streptococcus mutans, a major pathogen of dental caries, is considered to be one of the causative agents of infective endocarditis

PF-03084014 (IE). Two types of cell surface collagen-binding proteins, Cnm and Cbm, have been identified in the organism. The aim of the present study was to analyze these proteins as possible etiologic factors for IE. Materials and Methods The binding activities of S.mutans strains to collagen types I, III, and IV were analyzed relative FK228 molecular weight to the presence of Cnm and Cbm, as were their adhesion and invasion properties with human umbilical vein endothelial cells (HUVEC). In addition, distributions of the genes encoding Cnm and Cbm in S.mutans-positive heart valve specimens extirpated from IE and non-IE patients were analyzed by PCR. Results Most of the Cbm-positive strains showed higher levels of binding to type I collagen as well as higher rates of adhesion and invasion with HUVEC as compared to the Cnm-positive strains. Furthermore, the gene encoding Cbm was detected significantly more frequently in heart valve specimens from IE patients than from non-IE patients. Conclusions These results suggest that the collagen-binding protein Cbm of S.mutans may be one of the potential important factor associated with the pathogenesis of IE. Oral Diseases (2013) 19, 387-393″
“OBJECTIVE: To estimate response of diagnosis and symptom-based subtypes to sertraline treatment.

The time between the injury

and admission to the definiti

The time between the injury

and admission to the definitive trauma treatment center was an independent predictor of the likelihood of infection.

Conclusions: The time from the injury to operative debridement is not a significant independent predictor of the risk of infection. Timely admission to a definitive trauma treatment center has a significant beneficial influence on the incidence of infection after open high-energy lower extremity trauma.”
“A disruption of the vitamin A signaling pathway has been involved in age-related memory decline and hippocampal plasticity alterations. Using vitamin A deficiency (VAD), a nutritional model leading to a hyposignaling of the retinoid pathway, we have recently demonstrated that retinoic acid (RA), the active metabolite of vitamin A, Angiogenesis inhibitor is efficient to reverse VAD-induced spatial memory deficits and adult hippocampal neurogenesis alterations.

Besides, excess of glucocorticoids (GCs) occurring with aging is known to strongly inhibit hippocampal plasticity and functions and few studies report on the counteracting effects of RA signaling pathway on GCs action. Here, we have addressed whether the modulation of brain GCs availability could be one of the biological buy LDK378 mechanisms involved in the effects of vitamin A status on hippocampal plasticity and functions. Thus, we have studied the effects of a vitamin A-free diet for 14 weeks and a 4-week vitamin A supplementation on plasma and hippocampal corticosterone (CORT) levels in Wistar rats. We have also investigated corticosteroid binding globulin (CBG) binding capacity and 11beta-Hydrosteroid Dehydrogenase type 1 (11 beta-HSD1) activity, both important modulators of CORT availability at the peripheral and hippocampal levels respectively. Selleck AC220 Interestingly, we show that the vitamin A status regulates levels of free plasma CORT and hippocampal CORT levels, by acting through a regulation of CBG binding capacity and 11 beta-HSD1 activity. Moreover,

our results suggest that increased CORT levels in VAD rats could have some deleterious consequences on spatial memory, anxiety-like behavior and adult hippocampal neurogenesis whereas these effects could be corrected by a vitamin A supplementation. Thus, the modulation of GCs availability by vitamin A status is an important biological mechanism that should be taken into account in order to prevent age-related cognitive decline and hippocampal plasticity alterations.”
“We present experimental and simulation results for focused ion beam (FIB) milling of microchannels in lithium niobate in this paper. We investigate two different cuts of lithium niobate, Y- and Z-cuts, and observe that the experimental material removal rate in the FIB for both Y-cut and Z-cut samples was 0.

Minute ventilation and oxygen uptake were also measured Results:

Minute ventilation and oxygen uptake were also measured. Results: Root mean square (RMS) of the diaphragmatic EMG increased gradually without a plateau during incremental exercise, whereas the RMS increased initially and reached a plateau during constant work rate exercise. The RMS of the diaphragmatic EMG at the end of exercise was similar for both incremental and constant work rate exercise

(176 +/- 42 mu V vs. 184 +/- 39 mu V); these values were 70 and 73% of maximal values recorded over the study. Conclusions: The pattern of increase in neural respiratory drive during incremental exercise is different to that observed BV-6 during constant work rate exercise, but both exercise protocols are terminated when the patients achieve a similar but submaximal drive. Copyright (C) 2010 S. Karger AG, Basel”
“Objective: The research determining odors recognizable by children from Poland and Eastern Europe has not been widely described in the literature. The aim of the study was to determine the odors recognizable by Polish children which could be used in a screening olfactory test.

Methods: The study was performed in Children’s Hospital in Warsaw. Ninety-one children aged

from 2.9 years to 10 years (SD = 5.6 years) were examined, 85 (93.4%) of which completed a full olfactory test. Children were separated into three age groups. The olfactory test consisted of 21 ��-catenin signaling odors.

Results: The analysis of the results of all children’s examinations showed statistically significant influence of age on the number of odors identified Citarinostat mouse (p = 0.0001; r = 0.676).

The olfactory test score enabled identification of 6 odors to be included in the screening test: bubble gum, lemon, cola, mint, toffee, fish. Correct identification of at least 4 out of them was accepted as a norm and was achieved by 96.5% of children.

Conclusions: Olfactory evaluation is possible for children as young as 3 years old. Child’s age influences the number of odors recognized and the specific odors recognition

depends also on the home place geographic location and eating habits. Eastern European children most often recognized the following odors: bubble gum, lemon, cola, mint, toffee and fish, as so these odors were considered for olfactory screening test. The 6 odors test has good ability to generalize performance to olfactory status, has normative data and good validity and reliability, is fast, easy and inexpensive to administer. (C) 2012 Elsevier Ireland Ltd. All rights reserved.”
“Background: Some individuals with moderate/severe persistent asthma develop irreversible airway obstruction. These individuals present with dyspnoea, exercise intolerance and impaired quality of life (QOL), all of which could potentially be alleviated with exercise training.

(C) 2009 American Institute of Physics [doi: 10 1063/1 3236511]“

(C) 2009 American Institute of Physics. [doi: 10.1063/1.3236511]“
“Long-chain branching polypropylene (LCB-PP) was achieved by reactive extrusion in the presence of bifunctional monomer [1,6-hexanediol diarylate (HDDA)] and peroxide of dicumyl peroxide (DCP). Influences of HDDA and DCP concentrations on the branching efficiency were comparatively evaluated. Fourier transformed infrared spectroscopy

(FTIR) results indicated that the grafting reaction took place, and HDDA has been grafted on PP skeleton. In comparison with initial PP, some modified samples showed lower melt flow index because of a large number of LCB in their skeleton. Several rheology plots were used to investigate the rheological properties of the initial PP and modified PPs, and the rheological characteristics confirmed the LCB in modified PPs selleck skeleton. DSC results showed that the crystallization

temperatures of modified PPs were higher than those of initial PP and degraded PP, suggesting that the modified PPs had long-chain branched structure. The contrastive investigation in the rheology of modified Selleck CBL0137 PPS suggested that proper concentrations of HDDA and DCP were more beneficial to producing LCB during reactive extrusion. (C) 2009 Wiley Periodicals, Inc. J Appl Polym Sci 113: 2126-2135, 2009″
“A range of ion beam techniques have been used to fabricate a variety of photonic guiding structures in the well-known lithium niobate (LiNbO3 or LN) crystals that are of great importance in integrated photonics/optics. This paper reviews the up-to-date research Stem Cell Compound Library purchase progress of ion-beam-processed LiNbO3 photonic structures and reports on their fabrication, characterization,

and applications. Ion beams are being used with this material in a wide range of techniques, as exemplified by the following examples. Ion beam milling/etching can remove the selected surface regions of LiNbO3 crystals via the sputtering effects. Ion implantation and swift ion irradiation can form optical waveguide structures by modifying the surface refractive indices of the LiNbO3 wafers. Crystal ion slicing has been used to obtain bulk-quality LiNbO3 single-crystalline thin films or membranes by exfoliating the implanted layer from the original substrate. Focused ion beams can either generate small structures of micron or submicron dimensions, to realize photonic bandgap crystals in LiNbO3, or directly write surface waveguides or other guiding devices in the crystal. Ion beam-enhanced etching has been extensively applied for micro- or nanostructuring of LiNbO3 surfaces. Methods developed to fabricate a range of photonic guiding structures in LiNbO3 are introduced. Modifications of LiNbO3 through the use of various energetic ion beams, including changes in refractive index and properties related to the photonic guiding structures as well as to the materials (i.e.

Differently from the data obtained via the echo-Doppler approach,

Differently from the data obtained via the echo-Doppler approach, the tissue-Doppler method in patients without diastolic dysfunction showed a significant higher deceleration and isovolumetric relaxation times, with a lower Em velocity compared with the normotensive subjects. At the stepwise multiple regression analysis E/A ratio and E’/A’ values were related with left ventricular mass index and body mass index after correction for age. These data provide evidence that

diastolic dysfunction is of frequent detection in the earlier uncomplicated phases of the disease and that tissue Doppler detects an initial impairment of left ventricular relaxation in the patients in which at echo Doppler is still normal.”
“The objective of this study is Pevonedistat mw to observe the effect of high-mobility group protein B1 A Box (HMGB1 A) box on lung injury in mice with acute pancreatitis and its effect on the level of high-mobility group protein B1 (HMGB1) in lung, to explore the mechanism. A total of 60 male Institute of Cancer Research mice were randomly divided into control group (n = 30) and treatment group (n = 30). Severe acute pancreatitis

mice model was induced by 20% L-Arg intraperitoneal injection. The recombination HMGB1 A box was used in treatment after modeling. All the mice were killed under anesthesia at 24 and 48 h after the modeling injection. The level of HMGB1 and activity of myeloperoxidase (MPO) in lung were measured. The Nirogacestat mouse pathological changes of lung were observed. The level of HMGB1 in lung of A box treatment group decreased more significantly 24 h and 48 h after modeling compared with control group. The activity of MPO in lung of A box treatment group decreased more significantly compound screening assay 24 h after modeling compared with control group. The lung tissue pathologic score of A box treatment group decreased more significantly 48 h after modeling compared with control group. HMGB1 expression levels in the lungs were positively related to histological score of injured lung in acute pancreatitis. It indicates that HMGB1 A box is remarkably protective

to lung injury induced by acute pancreatitis.”
“Mandibular trauma is a common problem encountered by the craniofacial surgeon. Fractures in the edentulous mandible represent only 3% of these injuries, and optimal management is controversial. This problem is further compounded by malunion and malocclusion when fracture lines heal in incorrect positions. Even with recent advances in imaging and hardware systems, they remain difficult problems that often require multiple operations to obtain satisfactory results. We present a 69-year-old man with an edentulous mandibular fracture and concomitant malocclusion after a series of unsuccessful operations. By using computer-assisted design and manufacturing technology, we were able to plan osteotomies for correct anatomic positioning, which restored dental occlusion and facial aesthetics.