, 2008). Six modified Hagge corers ( Fleeger et al., 1988) of 30 cm length and 3.57 cm internal diameter (10 cm2 cross sectional area) were collected by SCUBA at each site. Samples were kept on ice immediately after collection and transferred

to the freezer on return to the laboratory, within 5 h. Cores were defrosted, and the top 5 cm was removed for examination of the Foraminifera (most living Foraminifera are found in this surface layer (Murray, 1991)), and the analysis of environmental factors. A subsample of the layer was homogenised and used for the determination of nitrogen and trace metal content. Obeticholic Acid molecular weight Sediments from the top 5 cm were first preserved in 70% ethanol and stained with Rose Bengal (24 h). Foraminifera were separated from the sediments by floatation using selleck products carbon tetrachloride (Murray, 1991) and 300 specimens (where possible) were mounted onto a slide for identification and determination of species diversity under a microscope at x 80 magnification. Specimens were separated

into live (stained) and dead individuals, and all were identified to species or morpho-species, where possible. Some Fissurina, Oolina and Lagena were identified only to genus, whilst bolivinids were identified as elongated or perforated. Species richness and diversity (Shannon Index; Magurran, 2004) were determined for each core. All foraminifera in the sediments were counted and abundance data were expressed as numbers/g sediment. After

the removal of the 300 Foraminifera, the sediment was dried (60 °C, 24 h), and sieved through meshes of 500 μm, 250 μm, 125 μm and 63 μm diameter in order to determine the granular size structure. The weight of sediment retained on each mesh was determined and the data were expressed as proportions. Mean sediment grain size (phi units) was calculated using GRADISTAT software ( Blott, 2010). While it could be argued that the removal of the Foraminifera from Tolmetin the samples might have impacted the size structure of the sediments, this would largely relate to the tests of dead specimens, which made up a maximum of 30% of the total individuals examined at each core. The nitrogen content (% N) of sediments was determined per site and not per core. Approximately 5 g of freshly defrosted sediment (i.e. before staining and extraction of Foraminifera and granulometry) from each core per site was dried (60 °C, 24 h), pooled and homogenised. A subsample was subsequently combusted in the presence of oxygen in order to determine the wt (%) of total nitrogen using a Eurovector EA CHN Analyser. Detection limits for the Analyzer were 0.1 wt (%). Calibration was performed using certified Eurovector standards, accepting a margin of error of 0.02%.

This study illustrates a recent movement of non-fishers (miners, salaried workers and non-fish traders) into fishing, following reports of high profits. The case of Cabuno does therefore support a need to invest in and develop alternative opportunities outside of fishing; prior to developing restrictions over ‘who is or is not’ allowed to fish [64]. Finally, with considerable industrial-scale fishing occurring all along the mainland coast, the importance of the Bijagós Islands as a regional ‘entry-point’ into SSF and a location where prosperous SSF activities PI3K inhibitor review are still possible, has increased through time. This generates

a very specific fisheries management problem. Prices for boat-ownership certificates and fishing licence documents are considerably higher in Guinea-Bissau (and the Bijagós archipelago) for non-nationals

(in-migrant) when compared to national (or local) citizens [47]. Raf inhibitor In ecological terms therefore, fish-stocks, biodiversity and ecosystem integrity may be threatened by uncontrolled SSF activity in this region; but in economic terms, the presence of these ‘foreign’ commercial SSF is highly prized. Unfortunately, with Guinea-Bissau׳s reputation for corruption, political violence, poor governance and weak institutional capacity, it seems highly unlikely that any resource-rent captured from SSF, in pursuit of a ‘wealth-based’ management strategy can or will be appropriately redistributed [14]. With severe political, climatic and economic uncertainties facing this West African region any prospects for non-fisheries development programmes appear bleak. With this in mind, an alternative governance trajectory might instead reflect upon the labour-intensiveness of SSF; developing effective strategies which focus upon poverty alleviation for example by improving health-care, insurance, education, infrastructure, access

check to land, micro-credit, communication and political free-will; while reducing susceptibility to accidents and HIV or AIDs-related illnesses within SSF communities [88], [7], [5] and [75]. While it is acknowledged that welfare approaches to fisheries management are not without fault; findings from this region suggest that any pursuit of wealth-based measures could be hugely catastrophic for those whose livelihoods depend upon SSF [14]. To conclude, West Africa׳s resources have for many years been misappropriated with resounding severe consequences incurred by millions [22], [3] and [23]. It is here argued that investing in misguided access-restrictions under the guise of wealth-based management would be akin to renewing this cycle.

Interventions to reduce inappropriate prescribing of antipsychotic medications to people with dementia resident in Bafilomycin A1 ic50 care homes may be effective in the short term, but longer-term, more robust studies are needed. For prescribing levels to be reduced in the long term, the culture and nature of care settings and the availability and feasibility of nondrug alternatives needs to be addressed. The authors thank Barbara Wider for invaluable assistance with translation and Alison Bethel for help with reference management. “
“Chemical pollutants, coastal zone destruction, habitat

loss, nutrient discharges, hypoxic zones, algal blooms and catastrophic overfishing have all heavily impacted life in our oceans (Bowen and Depledge, 2006). Major efforts are being made worldwide to manage and minimise these threats. However, one particular pollutant, light, is still permitted to flood into our seas almost unchecked. It is alarming that as the intentional and unintentional illumination of the coastal Dasatinib solubility dmso zone and nearshore environment increases unabated, we still have little idea of the extent to which intertidal and sublittoral ecosystems are being affected. There is also growing concern regarding

the introduction of light into the deep sea (Widder et al., 2005). Almost all living organisms are sensitive to changes in the quality and intensity of natural light in the environment (Longcore and Rich, 2004). This is such a widely distributed characteristic Ribose-5-phosphate isomerase that it seems likely to have arisen very early in

evolutionary history, possibly on several occasions. It might even suggest that the evolution of life in the oceans proceeded largely in the photic zone. Obviously, for algae and seaweeds, photosynthetic activity is critically dependent on available light, while in marine animals, tidal, daily, monthly and seasonal cycles in natural light intensity and quality are reflected in rhythmical fluctuations in behaviour and physiology that are appropriately tuned to the prevailing ecological circumstances (Depledge, 1984). Humans use the influence of light on several kinds of organisms to great advantage. For example, for centuries fishermen have deployed lanterns to attract fish to their nets, while modern day natural resource managers set out lights to attract larval fish to coral reefs to boost fish stocks and enhance biodiversity (Munday et al., 1998). There are numerous vivid accounts in the literature of people using their knowledge of light-entrained rhythms to reap rewards. South Pacific islanders for example, exploit moon phase spawning of polychaete worms to ensure bountiful harvests of eggs and sperm that are considered a culinary delicacy (Thorson, 1971). Light pollution of the sea has only become a really significant issue over the last ca. 50–80 years. It has been defined as the “degradation of the photic habitat by artificial light” (Verheijhen, 1985).

Comparing with the expansion stage, %CD41 increased during differentiation stage from 13% to 19% for G1, while for G2 raised from 13% to 35%, but only from 17% to 19% for G3 (Fig. 3C). Over differentiation stage, the total number of cells increased about 3.7 folds for G1 (corresponding to 6.3 ± 1.0 × 106 total cells), and 4.4 folds for G2 (corresponding to 19 ± 4.2 × 106 total

cells), selleck screening library but only about 1.3 for G3 (corresponding to 26 ± 13 × 106 total cells). Scanning electron microscopy analysis showed similar morphology of culture-derived platelet-like particles and human PB-derived platelets (Fig. 4A, right and left, respectively), demonstrating the ability of the current protocol to support the in vitro production of platelet-like particles. Likewise, transmission electron microscopy (TEM) analysis of culture-derived Mk (Fig. 4B) showed normal features of a mature Mks with demarcation membrane (dm) system, nucleus (N) and α-granules characteristic

of such mature Mk. Electron microscopy (SEM and TEM) imaging was performed on 3 different populations from G2 and for each culture, platelet-like particles (similar to the Fig. 4A) was identified in more than 10 microscopy images. PD-1 inhibitor Ploidy analysis (Fig. 5A) revealed that about 18% of culture-derived Mks have higher ploidy (>4 N). Moreover, forward (FCS) and side scatter (SCC) properties of such population are higher compared to the CD41+ cells with 2 N and 4 N DNA content (Fig. 5B). Mks generated from UCB, compared to human PB, were described to be smaller and have less ploidy; however, as reported Phenylethanolamine N-methyltransferase previously [13] and confirmed in the current study, these are still able to produce platelets-like particles. The current study presents a two-stage protocol aiming at effective megakaryocytic differentiation of UCB CD34+-enriched cells. The results identified distinct individual groups which elucidate the relation between FI-CD34+ and efficiency of Mk production. This information is valuable to

balance the proliferation and differentiation potential of CD34+ cells, when targeting efficient Mk production. The underlying phenomena for such balance should be actually based in cell population doublings, but FI-CD34+ is a tangible parameter easier to quantify. Several studies have reported production of Mk cells and platelets from HSC/HCP. For example, using UCB progenitors, a perfusion system was used to produce enough number of platelets in vitro for clinical transfusion (300–600 × 109) [16]. However, the drawback of aforementioned work was most of culture-derived platelets were activated in the absence of any agonists. Another study reported producing 44 ± 8.1 Mks/input HSC/HPC using human mPB cells through a complex 3-step culture; includes a cocktail comprised by 17 different cytokines and changes in pH and O2 tension during experiment [17].

In Experiment FB (top-left panel), TT is generally lower by 0.2–0.8 °C throughout the tropics, except for the strong localized warmings off the Central America and Baja California and the weak warming in the southeastern Pacific. In the regional experiments, locally-generated δTδT’s tend PR-171 cell line to be dominated by negative signals because T0zzT0zz tends to be negative above the pycnocline (Section 3.2.2; Fig. 4b). As discussed above, the locally-generated signals converge to the equator and propagate eastward along it. In the eastern-equatorial Pacific (EEPO), the

pycnocline rises near the surface so that upper-pycnocline water impacts TT there. Therefore, the part of the remotely-generated signals that impact δTδT in the EEPO are those that lie on the upper pycnocline. As a single measure of the impact of δκbδκb in the EEPO, we use δTδT averaged over the Niño-3 region ( δTN3;150°W– 90°W,5°S– 5°N). For solution FB, δTN3=-0.35°C. Individual contributions of the regional solutions to equatorial δTδT differ considerably, owing to the different, local, background

temperature and salinity selleck kinase inhibitor structures that generate them and their different ways of propagation. The largest contributions to negative δTN3δTN3 come from Solutions ESE and ENE (bottom and upper-middle right panels of Fig. 9), a consequence of their forcing regions having the largest overlap with the Niño-3 region. Interestingly, negative contributions from Solution EQE and EQW are much smaller, because the locally forced negative anomaly is balanced by the underlying, positive one that rises into the upper 50 m there (Fig. 8b). The contributions from Solutions ESW and ENW (bottom and upper-middle left panels of Fig. 9) are small because their near-surface, negative dynamical signals do not much propagate

to the eastern equatorial Pacific, and their positive dynamical signals partially cancel their negative spiciness signals (right panels of Fig. B.3b and Fig. B.4b). In Solutions NE (top-right panel of Fig. 9) and NW (not shown), there is a systematic warming   of TT in the EEPO, a consequence of the dynamical, warming signal rising to the surface there ( Fig. 7b and Fig. B.2b). In contrast, in Solutions SE and SW (not shown) δTδT in the EEPO is weak because C-X-C chemokine receptor type 7 (CXCR-7) their positive dynamical signal is balanced by a strong negative spiciness signal ( Fig. 6b and Fig. B.1b). The contribution from Solution SE is weakly negative because the negative spiciness signal dominates, and that from SW is weakly positive because the spiciness is somewhat weaker and dynamical signal is somewhat stronger ( Appendix B.1). It was surprising to us that the contributions to equatorial TT differ so much among the regional solutions, and that altogether they tend to cool, rather than warm, TT in the EEPO.

The pelagic mineralization rates will be more efficient and the phytoplankton uptake more than doubles (Meier et al., 2012a). As a result the oxygen levels are drastically reduced in large parts of the Baltic Sea (Fig. 5a). In the BSAP scenario the total load of nutrients from land and atmosphere was decreased by about one third. However, the reductions of external nutrient loads are not reflected in the internal dynamics, and the oxygen levels in large parts of the Baltic Sea do not improve significantly compared to present state. In the areas where the deep-water oxygen levels are critically low today the improvements are only slight or not evident

at all (Fig. 5b). This is an indication that climate-change NVP-LDE225 purchase impacts will reduce the effectiveness of the present abatement strategies during the simulation period. Worsened oxygen conditions in weakly stratified Selleck NVP-BGJ398 shallow areas are due to the temperature effect on oxygen solubility.

Both the BAU and the BSAP scenario indicate improvements in the Bothnian Bay and the Gulf of Finland. This is a response to increased mixing due to decreased stratification from the increased freshwater input from the northern rivers and Neva and slight increases in wind speed (Meier et al., 2011). Global modeling simulations show that if we reach a concentration of 850 ppm of CO2 in the atmosphere (equivalent with the IPCC SRES scenario A2, Fig. 6), we are facing an average pH decrease in oceanic surface CYTH4 waters of 0.4–0.5 pH units (Orr et al., 2005). This will result in a 100–150% increase in H+ concentration and a 50% reduction in CO32− concentration. The average surface pH of the ocean would be lower than it has been for more

than 20 million years (Feely et al., 2004). Baltic Sea model simulations (Edman and Omstedt, 2013 and Omstedt et al., 2009) indicate a change from stable conditions before industrialization and the beginning of acidification as CO2 concentrations in the atmosphere increases, with a likely dampened effect on the rate of acidification due to eutrophication (see discussion in the next section). However, results from Omstedt et al. (2012) illustrates that increased nutrient loads will not inhibit future Baltic Sea acidification. Regardless of the scenarios used the results implies that acidification will occur in the entire Baltic Sea. The impact of eutrophication on pH in the simulations was mainly by amplifying the seasonal pH cycle due to increased biological production and mineralization and reducing acidification in the anoxic deep layer. The projection of the surface water pH in the Eastern Gotland Basin (daily resolution) is illustrated in Fig. 7. Here the “business-as-usual” scenario (BAU-A2) is based on the IPCC SRES A2 scenario, together with increasing nutrient loads. In the simulations the seasonal pH cycle is amplified due to the increased nutrient loads which cause increased biological uptake of CO2 in surface waters.

For each animal at each PID, percentage relative

to the total number of uses (ipsilateral+contralateral+simultaneous) was calculated for ipsilateral (unimpaired) and contralateral (impaired) uses. An asymmetry score for each animal was calculated at each PID by the following formula: asymmetry score=(% of ipsilateral uses)−(% of contralateral uses). Animals with asymmetry score higher than 15 at PID 0 were discarded for statistical analysis. In the adhesive removal patch test, a small round adhesive paper (13 mm diameter) was placed on the inner portion of each wrist of the animal. One trial consisted in placing the adhesive papers and their subsequent removal by the animal. Four trials were applied at each PID, and trials were always separated Selleck PLX4032 by at least 5 min. Preference was evaluated, and in each trial the first side (ipsilateral Selleck GKT137831 or contralateral to the lesion) of removal was recorded. For each animal at each PID, percentage of contralateral preference relative to the total number of removals (four) was calculated.

Animals with preference to the right forelimb (more than 50% of first removal at pre-ischemic day) suffered focal ischemia in the left hemisphere (see Section 2.2.), and vice-versa. To check for lack of influence of whole experimental procedure in functional loss, untreated sham animals were also evaluated in adhesive test. To evaluate the plasmatic absorption of rutin after an i.p. injection, animals from R50 group were euthanized with CO2 2, 4, 6 or 8 h after the injection. Animals from the control group were also evaluated. Blood was collected by cardiac puncture with heparin and the plasma obtained by centrifugation at 12,000 g for 10 min. Plasma was acidified to pH 4.0 with phosphoric

acid. After acidification, methanol was added (1000 μl: 200 μl of plasma), and the sample was stirred for 1 min and centrifuged at 12,000 g for 10 min. Supernatant was collected, and the organic solvent was evaporated. Pellet was reconstituted with 200 μl of acidified water and analyzed using HPLC (LC-100, Shimadzu®) with reverse-phase column (RP-18, 5 μm, 4.0×250 mm2, Merck®), detector (SPD-M20A, Fenbendazole prominence diode array detector, Shimadzu®), loop injection of 20 μL, pump (LC 20 AT, prominence liquid chromatograph, Shimadzu®), injector (Rheodyne 7725i) and software LC Solution. The eluents were purified water adjusted to pH 3.2 with formic acid (A) and acetonitrile (B). The following solvent gradient was applied: from 100% A and 0% B to 80% A and 20% B within 10 min; from 80% A and 20% B to 75% A and 25% B within 5 min; from 75% A and 25% B to 70% A and 30% B within 10 min; from 70% A and 30% B to 50% A and 50% B within 10 min; and from 50% A and 50% B to 0% A and 100% B within 15 min (total analysis time: 45 min). Flow elution was 1 mL min−1; 20 μL of plasma samples were injected.

Based on several lines of evidence, Estes et al. (1998) concluded that killer whale (Orcinus orca) predation was the most parsimonious explanation for this decline. Garshelis and Johnson (1999) commented that equally compelling evidence Pictilisib for killer whale predation on otters existed for Knight Island. Common to both Knight Island and the Aleutians, there was no indication of reduced birthing or pup survival, few dead otters washed ashore (as they would in cases of disease, malnutrition, winter mortality,

or contamination), and body condition of otters indicated that food supplies were adequate ( Dean et al., 2000, Dean et al., 2002 and Laidre et al., 2006). In the Aleutians, only six killer whale attacks have been observed, and among these only three of the otters died (Hatfield et al., 1998). However, given the low probability of actually witnessing such brief events in this huge area, the three confirmed mortalities were extrapolated to an estimated 40,000 otters consumed by killer click here whales (Estes et al., 1998). It is now widely believed that killer whale predation reduced the Aleutian Islands’ otter population by more than 95% (Estes et al., 2005). Doroff et al. (2003, p. 55) called it “one of the most widespread and precipitous population declines for a mammalian carnivore in recorded history.”

Despite the rather scant observational evidence of the cause for this decline, when southwestern Alaska sea otters were proposed as a threatened population under the U.S. Endangered Species Act, killer whale predation was considered the most probable cause, with little support for other alternatives (U.S. Federal Register, 2004). Although intensive studies of both otters and killer whales have been conducted in PWS since

the early 1980s, the first attack was not witnessed until 1992 – by coincidence, shortly after the spill. All three observed killer whale attacks since then occurred at Knight Island, two of which were in Herring Bay, NKI (Hatfield et al., 1998). Additionally, in 2003, a killer whale was found dead in LaTouche Passage, south of Knight Island, with five sea otters in its stomach. This whale was identified as part of a pod whose range was centered in the Knight Island area (Vos et al., 2006). Killer whales could not only consume Leukotriene-A4 hydrolase several otters per day at Knight Island, but the risk of predation could drive otters to move to safer areas. Many scientists have moved beyond the question of whether killer whales began preying heavily on sea otters to why they did. Leading theories suggest that those killer whales that preferentially prey on marine mammals (rather than fish) have been forced to switch from diminishing stocks of harbor seals (Phoca vitulina) and Steller sea lions (Eumetopias jubatus) to much smaller, less-preferred sea otters. Although harbor seals are a preferred prey of most marine-mammal eating killer whales, in PWS whales prey equally on Dall’s porpoise (Phocoenoides dalli).

Another approach to reducing the high mortality rate of CRC is

to perform an inexpensive and non-invasive screen as part of a standard general physical examination for the appropriate population groups (e.g. persons over 50), which could detect a large fraction of patients who would normally be missed due to non-compliance. Improved fecal tests are being developed, for instance, based on molecular profiling of DNA such as the Exact Science Pre-Gen Plus™ (Berger et al., 2006); however, such tests have not been widely accepted by the medical community, potentially due to the emphasis on home-collection of fecal samples (Woolf, 2004). Yet diagnosing CRC at an early stage is indispensable as the 5-year survival rate is around 90% when caught at the localized SB431542 molecular weight stage (SEER Summary Staging) and drops to 70% with regional metastasis and 12% with distant metastasis (Howlader et al., 2012). Therefore, an early, non-invasive screen for CRC which can complement the colonoscopy is urgently needed. In contrast to fecal based CRC screening, blood testing based on detection of multiple biomarkers provides a minimally-invasive, more patient friendly method of pre-screening for CRC. One such approach is based on detection of aberrant methylation of CpG-islands (CGI-methylation) in freely circulating Small molecule library DNA in blood. Epigenomics is developing

Epi ProColon, a blood-based test based on detection of methylation markers in Septin9 (Toth et al., 2012). Serum proteins and autoantibodies against tumor-associated antigens (TAAs) in blood also comprise a potential source of valuable CRC biomarkers. A 2011 review found 63 studies on the serological diagnosis of CRC with more than 50 TAAs oxyclozanide and other serum protein biomarkers in development (Creeden et al., 2011). Autoantibodies to TAAs have been detected in patient’s blood even in the

early stages of cancer (Chapman et al., 2008). Furthermore, autoantibody biomarkers have several advantages over other serum biomarkers, including long-term stability and “the inherent amplification of signals provided by the host’s own immune system to low levels of tumor-associated antigens in early disease” ( Anderson and LaBaer, 2005 and Storr et al., 2006). However, any single autoantibody biomarker rarely exceeds 15% diagnostic sensitivity ( Zhang et al., 2003, Casiano et al., 2006 and Belousov et al., 2008), thereby highlighting the need to discover and clinically validate large panels or signatures of TAAs, in multiplex, as well as to combine autoantibodies with other serum biomarker types such as circulating proteins, to achieve both sensitive and specific cancer diagnosis. In the genomics realm, highly parallelized and multiplexed bio-assay technologies such as high density DNA microarrays/micro-bead arrays (Fodor et al., 1991, Chee et al., 1996 and Gunderson et al., 2004), massively parallel DNA sequencing (Margulies et al., 2005 and Bentley et al., 2008), microfluidic chips (Dettloff et al.

Baudienst), niejednokrotnie ponad 12 godzin dziennie. Poza tym prowadzono systematyczną akcję germanizacyjną dzieci polskich. Na podstawie odnalezionych dokumentów wiadomo, że dokonywano „rabunku” dzieci polskich, zwłaszcza uznanych jako „rasowo-wartościowe”. Zgodnie z planem Himmlera i Urzędu ds. Rasowo-Politycznych

NSDAP objęto zarządem wszystkie zakłady opiekuńcze i wychowawcze, zakazując używania języka polskiego. Odbierano dzieci pochodzące z rodzin mieszanych, pozbawione rodziców (aresztowanych lub zmarłych), będących pod opieką innych członków rodziny i kierowano je do niemieckich zakładów wychowawczych (m.in. w Poznaniu, Puszczykowie i Kaliszu) lub do niemieckich rodzin zastępczych. Dla zatarcia śladów tej zbrodniczej działalności dzieciom tym zmieniano daty urodzenia oraz imiona i nazwiska na germańskie find more (instytucja Lebensborn E.V.). Chróścielewski [13] wskazywał, że te indywidualne działania były mniej buy Z-VAD-FMK znane. Powszechnie natomiast wiadomo o masowym wysiedleniu ludności Zamojszczyzny

w 1943 roku, w tym około 30 tysięcy dzieci, z których 4450 po przebadaniu rasowym wysłano do Rzeszy celem zniemczenia. Wiele zginęło w specjalnych obozach o zaostrzonych rygorach, m.in. w Łodzi, gdzie czynny był Polen Jugendverwahrlager der Sicherheitspolizei in Litzmanstadt. Od 8. roku dzieci zobowiązane były do pracy. Niewykonanie zadań karano pozbawieniem posiłku, aresztem lub chłostą. Powszechne były wyniszczające choroby, kończące się zgonem. Przez obóz w Łodzi przeszło około 12 tysięcy dzieci [13], w czasie wyzwolenia uratowano 600–800 dzieci, z czego część ciężko chorych zmarła. Oddziały dziecięce były również w obozach koncentracyjnych w Majdanku i Oświęcimiu [13]. Całkowitej eksterminacji podlegały dzieci żydowskie. Trudno dziś

uwierzyć, że na zebraniu lekarzy Selleckchem Pembrolizumab można było usłyszeć (Matthias Mayer, Inowrocław, 22.07.1944), iż „nie należy leczyć dzieci polskich […] leczenie ich jest niepotrzebne i nie wymaga tego narodowo-socjalistyczna polityka narodowościowa Niemiec” [wg 12]. Profesor Chróścielewski jednoznacznie podkreślał: „Zbrodnia popełniona na niewinnych dzieciach stanowi jedną z najciemniejszych kart historii II wojny światowej. Jest hańbą XX wieku” [18]. Jego rówieśnik, z tej samej poznańskiej uczelni, pediatra profesor Olech Szczepski stwierdzał, że „zbrodnie ostatniej wojny, popełniane w koncentracyjnych obozach Oświęcimia, Dachau i Buchenwaldu odebrały lekarzom ich moralny immunitet” [19]. Ta grupa prac Chróścielewskiego pozwala przypomnieć owe bolesne, a historycznie ważne dla narodu polskiego wydarzenia, szczególnie w obliczu sporadycznie pojawiających się haseł profaszystowskich. Wizja społecznej roli medycyny sądowej u Chróścielewskiego znalazła wyraz w wielokierunkowym rozwijaniu tej problematyki, zwłaszcza w odniesieniu do młodzieży. Analizował związki utonięć młodzieży z alkoholem, narastający problem urazowości [14] i samobójstw wśród młodzieży [15].